Evaluation of the Antioxidant and Antimicrobial Activity of Genista quadriflora (G.Q) Plant Extracts

Sozial- und Praventivmedizin Pub Date : 2021-01-01 DOI:10.3166/PHYTO-2021-0249

Assia Berrani, Moncef Boufellous, H. Frikh, M. Aouji, L. A. Lrhorfi̇

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Abstract

In the framework of the valorization of the plants of Morocco and in order to optimize the subsequent use of the plant Genista quadriflora (Fabaceae) in Morocco, extracts were prepared from the plant using solvents of increasing polarity to emphasize their antioxidant potency and antimicrobial activity. The evaluation of the antioxidant power, using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging method, indicated by qualitative analysis of the extracts either by thin layer chromatography (TLC) or by color reaction, that the extracts (aqueous and methanol) have an antioxidant effect varies between 70% and 80%. As regards the 50% inhibitory concentration (IC50) scavenging values of the DPPH radical, the two extracts showed an inhibitory activity with IC50 at around 12.5 mg/ml. The antimicrobial activity was evaluated by the agar diffusion method against Staphylococcus aureus, Escherichia coli, Candida albicans and Klebsiella pneumoniae. This evaluation showed that the methanol extracts are the most active for all the strains tested and revealed a significant antibacterial activity against Escherichia coli.

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四连藤(G.Q)植物提取物抗氧化和抗菌活性的评价

在摩洛哥植物增值的框架下，为了优化摩洛哥植物Genista quadriflora (Fabaceae)的后续利用，使用极性增加的溶剂从该植物中提取提取物，以强调其抗氧化能力和抗菌活性。采用1,1-二苯基-2-吡啶肼基(DPPH)自由基清除法对提取物进行抗氧化能力评价，通过薄层色谱(TLC)定性分析和显色反应定性分析表明，提取物(水溶液和甲醇)的抗氧化效果在70% ~ 80%之间。对于DPPH自由基的50%抑制浓度(IC50)清除率，两种提取物的IC50均在12.5 mg/ml左右。采用琼脂扩散法对金黄色葡萄球菌、大肠杆菌、白色念珠菌和肺炎克雷伯菌进行抑菌活性评价。结果表明，甲醇提取物对所有菌株的抑菌活性最高，对大肠杆菌具有显著的抑菌活性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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