Evaluation of the Antimicrobial Activity ZnO/Ampicillin Nanocomposite Against Sensitive and Resistant Strains: Role of γ-Irradiation on Its Biological Activity

Z. Tawfik, A. Badawi, H. Ahmed, Mohamed Lethy, Z. Abdeen
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引用次数: 2

Abstract

ABSTRACT In this study the ampicillin trihydrate was modified to ZnO/ampicillin nanocomposite to improve its effectiveness as a bactericidal agent against sensitive and resistant bacterial strains. The antimicrobial activity of the standard ampicillin trihydrate and ZnO/ampicillin nanocomposite on three pathogenic bacterial strains, Gram-negative short rods, Salmonellae typhimurium, and Gram-positive cocci Staphylococcus aureus were investigated. These strains were recommended in the British Pharmacopeias to be used as the most sensitive strains for the antibiotic sensitivity test of ampicillin. Another strain of Staphylococcus aureus referred as ampicillin resistant clinical isolate was also used. Stock cultures were maintained on nutrient agar slants at 40°C until required. Gamma-irradiation unit of Co60was used for irradiation of the standards ampicillin trihydrate and ZnO ampicillin nanocomposite at the sterilizing dose 25 kGy. The antibacterial activity of the standard ampicillin was significantly en...
ZnO/氨苄西林纳米复合材料对敏感和耐药菌株的抑菌活性评价:γ辐照对其生物活性的影响
摘要:本研究将三水合氨苄西林修饰为ZnO/氨苄西林纳米复合材料,以提高其作为敏感和耐药菌株的杀菌剂的有效性。考察了三水合氨苄西林和氧化锌/氨苄西林纳米复合材料对革兰氏阴性短杆菌、鼠伤寒沙门氏菌和革兰氏阳性金黄色葡萄球菌3种病原菌的抑菌活性。这些菌株在英国药典中被推荐作为氨苄西林抗生素敏感性试验的最敏感菌株。另一株金黄色葡萄球菌被称为耐氨苄西林临床分离株也被使用。在40°C的营养琼脂斜面上保持存量培养,直到需要。采用co60的γ辐照单元对标准三水合氨苄西林和氧化锌氨苄西林纳米复合材料进行灭菌剂量为25 kGy的辐照。标准氨苄西林的抑菌活性显著提高。
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