Evaluation of the Antimicrobial Activity ZnO/Ampicillin Nanocomposite Against Sensitive and Resistant Strains: Role of γ-Irradiation on Its Biological Activity
Z. Tawfik, A. Badawi, H. Ahmed, Mohamed Lethy, Z. Abdeen
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引用次数: 2
Abstract
ABSTRACT In this study the ampicillin trihydrate was modified to ZnO/ampicillin nanocomposite to improve its effectiveness as a bactericidal agent against sensitive and resistant bacterial strains. The antimicrobial activity of the standard ampicillin trihydrate and ZnO/ampicillin nanocomposite on three pathogenic bacterial strains, Gram-negative short rods, Salmonellae typhimurium, and Gram-positive cocci Staphylococcus aureus were investigated. These strains were recommended in the British Pharmacopeias to be used as the most sensitive strains for the antibiotic sensitivity test of ampicillin. Another strain of Staphylococcus aureus referred as ampicillin resistant clinical isolate was also used. Stock cultures were maintained on nutrient agar slants at 40°C until required. Gamma-irradiation unit of Co60was used for irradiation of the standards ampicillin trihydrate and ZnO ampicillin nanocomposite at the sterilizing dose 25 kGy. The antibacterial activity of the standard ampicillin was significantly en...