Stability of Blood Glucose in Fluorinated Plasma and Plane Tube Serum

Abstract

Introduction: In 1941 Sodium fluorite containing tubes was developed. Since then these tables were used in the clinical laboratory for measurement of glucose. Sodium fluoride is a useful agent that inhibits glycolysis. The glucose level in the unpreserved blood sample can decrease by 5-7% per hour due to glycolysis. Methods: The objective to evaluate the stability of blood glucose in fluorinated plasma and plane tube serum. The Blood samples obtained from 30 participants and which pour into two different (fluorinated and plane) tube. Plasma and serum separated and storage within 25-30 Minute of the collection on average. Separated samples were aliquoted into eppendorfs and stored in the different cool box at 4-8°C. Glucose concentrations were analyzed by glucose oxidase method using the SELECTRA Pro-M Auto-analyzer at 0hours (within 30 min), 24hours and 48 hours. Paired sample t-test performed significantly at a P-value of less than 0.05. Results: Results show that the rate at which glucose concentration changed with time varies in fluorinated plasma and plane tube serum, the average mean difference of glucose 1.2 mg/dl, 9.2 mg/dl, and 3.32 mg/dl, 3.11 mg/dl in plasma and serum from 0 h-24 h and 0 h-48 h respectively. A statistically significant correlation (p<0.05) found with times of measurement. Glucose levels slightly reduce in plasma than serum within 24 h but comparatively more glycolysis occurs in plasma than serum after 24 hour. Conclusion: This study tries to find out a suitable agent for assessing blood glucose levels when the analysis delayed. Sodium fluoride is an effective agent for delay glucose analysis.