Detection of Cpy-2, Fks and Erg 11 Genes in Fungal Isolates from Sars Cov-2 Individual in Ibadan, Oyo State, Nigeria

IF 1.1 Q4 ALLERGY
Bamigbola F
{"title":"Detection of Cpy-2, Fks and Erg 11 Genes in Fungal Isolates from Sars Cov-2 Individual in Ibadan, Oyo State, Nigeria","authors":"Bamigbola F","doi":"10.37191/mapsci-2582-6549-4(1)-042","DOIUrl":null,"url":null,"abstract":"A considerable number of fungal strains have developed resistant to various available antifungal agents due to CPY, FKS and or ERG11 genes complicating coinfection cases of SAR COV-2 virus. Therefore, this study sought to isolate, identify azole and polyene resistant genes in fungal pathogens isolated from confirmed SARS-CoV-2 individual in Oyo State, Nigeria. Nasopharyngeal samples were collected from symptomatic and asymptomatic SARS-CoV-2 infected adult from September, 2020 to April, 2021. Samples were cultured on Sabouraud Dextrose Agar at room and at 37 oC temperature for 7days. Identification of the fungal isolates were performed using MALDITOF MS VITEK. Antifungal Susceptibility Testing (AFST) were performed using Kirby bauer disc diffusion method. The resistant genes in fugal isolates were determined by Polymerase Chain Reaction with specific primers and resistant genes were amplified using agarose gel electrophoresis. Out of 63(15.8%) fungal isolates recorded from 400 samples collected, Asipergillus flavus 11(17.5%), Aspergillus niger 9(14.3%), Candida albicans 7(11.1%), Candida guillermondii 2(3.2%), Candida parapsilosis 2(3.2%), Candida famata 2(3.2%), Candida tropicalis 5(7.9%) and Lodderomyces elongisporus 25(39%) having highest frequency were recorded respectively. Nystatin (84.1%) had highest susceptibility testing and Ketoconazole (39.7%) had the least phenotypically. 10 (52.6%) isolates possessed CPY gene, 8(42.1%) isolates carried FKS gene, 9(47.4%) isolates had ERG11 gene molecularly.","PeriodicalId":15543,"journal":{"name":"Journal of Cutaneous Immunology and Allergy","volume":null,"pages":null},"PeriodicalIF":1.1000,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Cutaneous Immunology and Allergy","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.37191/mapsci-2582-6549-4(1)-042","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"ALLERGY","Score":null,"Total":0}
引用次数: 0

Abstract

A considerable number of fungal strains have developed resistant to various available antifungal agents due to CPY, FKS and or ERG11 genes complicating coinfection cases of SAR COV-2 virus. Therefore, this study sought to isolate, identify azole and polyene resistant genes in fungal pathogens isolated from confirmed SARS-CoV-2 individual in Oyo State, Nigeria. Nasopharyngeal samples were collected from symptomatic and asymptomatic SARS-CoV-2 infected adult from September, 2020 to April, 2021. Samples were cultured on Sabouraud Dextrose Agar at room and at 37 oC temperature for 7days. Identification of the fungal isolates were performed using MALDITOF MS VITEK. Antifungal Susceptibility Testing (AFST) were performed using Kirby bauer disc diffusion method. The resistant genes in fugal isolates were determined by Polymerase Chain Reaction with specific primers and resistant genes were amplified using agarose gel electrophoresis. Out of 63(15.8%) fungal isolates recorded from 400 samples collected, Asipergillus flavus 11(17.5%), Aspergillus niger 9(14.3%), Candida albicans 7(11.1%), Candida guillermondii 2(3.2%), Candida parapsilosis 2(3.2%), Candida famata 2(3.2%), Candida tropicalis 5(7.9%) and Lodderomyces elongisporus 25(39%) having highest frequency were recorded respectively. Nystatin (84.1%) had highest susceptibility testing and Ketoconazole (39.7%) had the least phenotypically. 10 (52.6%) isolates possessed CPY gene, 8(42.1%) isolates carried FKS gene, 9(47.4%) isolates had ERG11 gene molecularly.
尼日利亚奥约州伊巴丹地区Sars Cov-2个体真菌分离株Cpy-2、Fks和Erg - 11基因的检测
由于合并感染SAR COV-2病毒的CPY、FKS和/或ERG11基因,相当多的真菌菌株已经对各种可用的抗真菌药物产生耐药性。因此,本研究试图从尼日利亚Oyo州确诊的SARS-CoV-2个体分离的真菌病原体中分离并鉴定唑和多烯耐药基因。于2020年9月至2021年4月采集有症状和无症状SARS-CoV-2感染成人的鼻咽样本。在室温和37℃条件下,在Sabouraud葡萄糖琼脂上培养7天。采用MALDITOF MS VITEK对分离真菌进行鉴定。采用Kirby bauer圆盘扩散法进行抗真菌药敏试验(AFST)。采用特异性引物聚合酶链式反应法测定菌株的耐药基因,并用琼脂糖凝胶电泳法扩增耐药基因。从400份样品中分离出63株真菌(15.8%),其中黄曲霉11株(17.5%)、黑曲霉9株(14.3%)、白色念珠菌7株(11.1%)、吉勒蒙念珠菌2株(3.2%)、副假丝酵母2株(3.2%)、famata念珠菌2株(3.2%)、热带念珠菌5株(7.9%)和长孢loderomyces elongisporus 25株(39%)的频率最高。制霉菌素敏感性最高(84.1%),酮康唑敏感性最低(39.7%)。10株(52.6%)携带CPY基因,8株(42.1%)携带FKS基因,9株(47.4%)携带ERG11基因。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
0.60
自引率
10.00%
发文量
69
审稿时长
12 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信