In vivo effects of methamphetamine on brown fat reactive oxygen species and mitochondria

Nikki Bortell, Julia A. Najera, M. Sanchez-Alavez, M. C. Marcondes
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引用次数: 6

Abstract

Methamphetamine (commonly known as Meth) is a highly addictive drug of abuse, which causes a potentially lethal increase in core body temperature, or hyperthermia. We have recently found that Meth-induced hyperthermia has a significant participation of the thermogenic brown adipose tissue,1 and can be prevented by a pretreatment with the antioxidant N-acetyl cysteine.1 For this publication, we labeled reactive oxygen species, such as superoxide, in vivo, by injecting C57Bl/6 mice with dihydroethidium; we then harvested and processed interscapular brown adipose tissue (for methods, see ref. 1). Reactive oxygen species were visualized in brown fat and found to be largely associated with mitochondria (Slide 1). In the slide, dihydroethidium-labeled superoxide is seen as red; the mitochondrial marker TOMM20 is seen as yellow; the cytoskeleton F-actin marker Phalloidin Alexa488 is seen as green; and the DNA marker DAPI is seen in blue. Meth depleted superoxide in brown-fat mitochondria, in correlation with the loss of TOMM20-labeled mitochondria. These changes were detectable in brown fat as early as 15 minutes after the injection of the drug, with a peak at 1 hour following injection, which is seen in Slide 1. The N-acetyl cysteine pretreatment prevented the loss of TOMM20 induced by Meth, but did not restore the Meth-depleted superoxide storages in mitochondria. Overall, this slide shows that Meth impacts the mitochondrial storages of superoxide, as well as mitochondrial integrity, in brown adipose tissue. The functional importance of these observations remains to be established and requires further studies.
甲基苯丙胺对棕色脂肪活性氧和线粒体的体内影响
甲基苯丙胺(俗称冰毒)是一种极易上瘾的滥用药物,它会导致核心体温升高或高热,可能致命。我们最近发现,冰毒诱导的热疗有产热棕色脂肪组织的显著参与,并且可以通过抗氧化剂n -乙酰半胱氨酸预处理来预防在这篇文章中,我们通过给C57Bl/6小鼠注射二氢乙啶,在体内标记活性氧,如超氧化物;然后,我们收获并处理肩胛间棕色脂肪组织(方法见参考文献1)。在棕色脂肪中可见活性氧,并发现其与线粒体主要相关(幻灯片1)。在幻灯片中,二氢乙二酸标记的超氧化物被视为红色;线粒体标记TOMM20呈黄色;细胞骨架f -肌动蛋白标记物Phalloidin Alexa488呈绿色;DNA标记DAPI是蓝色的。甲基安非他明减少了棕色脂肪线粒体中的超氧化物,这与tomm20标记的线粒体的丢失有关。这些变化早在注射药物后15分钟就可以在棕色脂肪中检测到,在注射后1小时达到峰值,如图1所示。n -乙酰半胱氨酸预处理可以防止甲基化诱导的TOMM20丢失,但不能恢复甲基化后线粒体中超氧化物的储存。总的来说,这张幻灯片显示甲基安非他命影响了棕色脂肪组织中超氧化物的线粒体储存,以及线粒体的完整性。这些观察的功能重要性仍有待确定,需要进一步研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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