Spectrophotometric Determination of Zinc in Pharmaceutical Medication Samples Using 8-Hydroxyquinoline Reagent

International Journal of Chemistry Pub Date : 2019-10-20 DOI:10.5539/ijc.v12n1p29

S. Najim, M. A. A. Hameed, M. Al-Shakban, Tahseen Saddam Fandi

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引用次数: 2

Abstract

Simple, rapid, cheap and sensitive spectrophotometric method has been described for the determination of zinc in pharmaceutical samples. The method is based on the formation of zinc- 8-Hydroxy quinoline chelate, the maximum absorption (lmax) at 384 nm. The method obeyed Beer's law in the range 1-5 mg/mL and the corresponding molar absorptivity value is 0.01578 ´ 103 L.mol-1.cm-1. The Sandell sensitivity values of limits of detection (LOD) and quantification (LOQ) was 0.381mg/mL and 1.156 mg/mL respectively. The recovery percentage of zinc was found 98.00 %, 98.96 %,99.91%, 97.50%, 98.5% and 99.30 % for (Capsule-13 mg), (Tablet-20 mg), (Tablet-40 mg),(Capsule-50 mg), (Capsule-50 mg) and (insulin vial-0.025 mg) respectively. All variable parameters has been optimized according to ICH guidelines. The limiting concentrations of some cations for interference by Mn(II), Fe(II), Co(II), Ni(II), Cu(II), Cd(II),Sn(II) ,Pb(II), Mg(II), Ca(II) and Ba(II) are reported. The method accuracy was established by comparison with conventional flame atomic absorption spectrometric method by using t-test, ttab.= 2.571, tcal. =0.3231 at 95% confidence level, indicating the absence of systematic errors.

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8-羟基喹啉试剂分光光度法测定药物样品中的锌

介绍了一种简单、快速、廉价、灵敏的分光光度法测定药品样品中锌的方法。该方法是基于锌- 8-羟基喹啉螯合物形成的，最大吸收(lmax)在384 nm处。该方法在1 ~ 5 mg/mL范围内符合比尔定律，相应的摩尔吸光度值为0.01578 × 103 L.mol-1.cm-1。检出限(LOD)和定量限(LOQ)的Sandell灵敏度分别为0.381mg/mL和1.156 mg/mL。锌的回收率分别为(13 mg)、(20 mg)、(40 mg)、(50 mg)、(50 mg)和(胰岛素瓶-0.025 mg)的98.00%、98.96%、99.91%、97.50%、98.5%和99.30%。所有可变参数均按照ICH指南进行了优化。报道了Mn(II)、Fe(II)、Co(II)、Ni(II)、Cu(II)、Cd(II)、Sn(II)、Pb(II)、Mg(II)、Ca(II)和Ba(II)干扰阳离子的极限浓度。采用t检验与传统火焰原子吸收光谱法进行比较，确定了方法的准确性。= 2.571, local。=0.3231, 95%置信水平，表明不存在系统误差。

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International Journal of Chemistry

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