Iron(III) -chelating resins X. Iron detoxification of human plasma with iron(III) -chelating resins

M. Feng, L. van der Does, A. Bantjes
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引用次数: 9

Abstract

Iron detoxification of human blood plasma was studied with resins containing desferrioxamine B (DFO) or 3-hydroxy-2-methyl-4(1H)-pyridinone (HMP) as iron(III)-chelating groups. The behaviour of four resins was investigated: DFO-Sepharose, HMP-Sepharose and crosslinked copolymers of 1-(β-acrylamidoethyl)-3-hydroxy-2-methyl-4(1H)-pyridinone (AHMP) with 2-hydroxyethyl methacrylate (HEMA) and of AHMP with N,N-dimethylacrylamide (DMAA). The efficiency of iron detoxification of plasma of the resins was mainly dependent on the affinity of the ligands and the hydrophilicity of the resins. The results of a stability study in phosphate-buffered saline at a physiological pH indicated that AHMP-DMAA was the most stable resin, whereas the Sepharose gels had a relatively lower stability. Experiments with the AHMP-DMAA resin showed that the resin was able to remove iron from plasma with different iron contents, and from plasma poisoned with FeCl3, iron(III) citrate or transferrin. A rapid removal from free serum iron was observed, whereas iron from transferrin was removed slowly afterwards. Only the overload iron was removed since in all cases the normal serum iron level of ca. 1 ppm was obtained.

铁(III)螯合树脂。铁(III)螯合树脂对人体血浆铁的解毒作用
用含去铁胺B (DFO)或3-羟基-2-甲基-4(1H)-吡啶酮(HMP)为铁(III)螯合基团的树脂研究了人血浆中铁的解毒作用。研究了四种树脂:DFO-Sepharose, HMP-Sepharose以及1-(β-丙烯酰胺乙基)-3-羟基-2-甲基-4(1H)-吡啶酮(AHMP)与2-甲基丙烯酸羟乙酯(HEMA)和AHMP与N,N-二甲基丙烯酰胺(DMAA)交联共聚物的行为。树脂的等离子体解毒效率主要取决于配体的亲和力和树脂的亲水性。在生理pH下磷酸盐缓冲盐水中的稳定性研究结果表明,AHMP-DMAA是最稳定的树脂,而Sepharose凝胶的稳定性相对较低。AHMP-DMAA树脂的实验表明,该树脂能去除不同铁含量的血浆中的铁,也能去除FeCl3、柠檬酸铁(III)和转铁蛋白中毒的血浆中的铁。观察到游离血清铁的快速去除,而转铁蛋白中的铁随后缓慢去除。只有超载铁被去除,因为在所有情况下,正常血清铁水平约为1 ppm。
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