Analysis of Hydroquinone and Kojic Acid Content in Three Brand Body Lotions Using High Performance Liquid Chromatography

JURNAL ILMIAH SAINS Pub Date : 2023-06-10 DOI:10.35799/jis.v23i1.45703

Yessy Dwi Anggraini, Khoirul Anwar, A. Budiarti

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Abstract

Treatment of hyperpigmentation can be done with several compounds, including a combination of hydroquinone and kojic acid in cosmetic preparations that are considered safe and effective. This study aims to analyze the levels of hydroquinone and kojic acid in three trademarks of body lotion using HPLC. The study used HPLC at a wavelength of 283 nm. The stationary phase used was C18, and the mobile phase was a mixture of acetonitrile:aquabidest (20:80, v/v) with a flow rate of 1.0 mL/min. Validation tests were carried out, including linearity, sensitivity (LOD and LOQ), selectivity, precision, and accuracy. The analytical method was applied to 3 body lotion preparations. The linearity test of hydroquinone and kojic acid with a value of r = 0.999; Hydroquinone LOD 0.274 g/mL; LOQ of hydroquinone 0.913 g/mL; LOD of kojic acid 0.420 g/mL; LOQ of kojic acid 1,401 g/mL; selectivity test 1.5; the precision test of hydroquinone and kojic acid resulted in the value of %RSD 2%; hydroquinone accuracy 99.200-100.450%; kojic acid 99.454-100.450%. The content of hydroquinone body lotion brand A, 0.854%, B 0.470%, and C 1.557%, kojic acid content in body lotion brand A 0.823%, B 0.510%, and C 1.497%. The results showed that all the results of the validation test fulfill the requirements of the validation parameters and the results of the analysis of the content of hydroquinone and kojic acid in the three trademarks of body lotion fulfill the requirements. Keywords: Body lotion; hydroquinone levels; kojic acid

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高效液相色谱法分析三种品牌身体乳中对苯二酚和曲酸的含量

色素沉着的治疗可以用几种化合物来完成，包括在化妆品制剂中的对苯二酚和曲酸的组合，被认为是安全有效的。本研究旨在利用高效液相色谱法分析三种身体乳商标中对苯二酚和曲酸的含量。采用高效液相色谱法，波长为283 nm。固定相为C18，流动相为乙腈:水相(20:80,v/v)，流速为1.0 mL/min。进行了验证试验，包括线性、灵敏度(LOD和LOQ)、选择性、精密度和准确度。将分析方法应用于3种身体乳制剂。对苯二酚与曲酸的线性关系为r = 0.999;对苯二酚LOD 0.274 g/mL;对苯二酚的定量限为0.913 g/mL;曲酸LOD 0.420 g/mL;曲酸定量限1401 g/mL;选择性检验1.5;对苯二酚和曲酸精密度试验的RSD为2%;对苯二酚精度99.200-100.450%;曲酸99.454-100.450%。A牌对苯二酚类身体乳含量为0.854%，B为0.470%，C为1.557%，A牌身体乳中曲酸含量为0.823%，B为0.510%，C为1.497%。结果表明，验证试验结果均满足验证参数的要求，3个身体乳商标对苯二酚和曲酸含量分析结果均符合要求。关键词:沐浴露;对苯二酚的水平;曲酸

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