Poultry By-Product Meal as a Potential Protein Source in Broiler Diets with Exogenous Protease Supplementation

Heshani Amalka Vithana, S. P. Macelline, S. R. Nawarathne, D. Jayasena, M. Yu, Eunsoo Seo, Mangala Amarsinghe, M. N. Nambapana, J. Cho, J. Heo
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Abstract

The objective of this study was to investigate the effect of exogenous protease supplementation in diets formulated with poultry by-product meal on growth performance, small intestine magnitudes, and meat physiochemical characters in broiler chickens from 21 to 35 days post-hatch. A total of 120, one-day-old "Arbor Acres Plus" broiler chickens (male: female - 50:50) were allocated one of two dietary treatments to give six replicates and ten birds per cage. Two dietary treatments included a control diet (CON) and a diet supplemented with protease (CON+Pro). At day 35, body weight and feed intakes were measured to calculate the feed conversion ratio for the entire experiment period. Two birds from each pen were euthanized to measure the relative lengths and relative weights of three small intestine sections and meat samples were collected for physiochemical characteristic analyses at 35 days post-hatch. Exogenous protease supplementation did not influence ( P >0.05) growth performance but showed a tendency to improve FCR ( P =0.082). Protease supplementation showed a tendency to reduce proximal small intestine length ( P =0.091). Broilers offered dietary treatments influenced minced meat color where protease supplementation resulted in lower CIE b * ( P <0.001) colorimetric value for yellowness and showed a significant trend ( P =0.059) on reducing meat redness CIE a * . In conclusion, the addition of exogenous protease to a broiler diet formulated with poultry by-product meal did not affect the growth performance, small intestine magnitudes, and meat physiochemical parameters (except CIE b * ) in broiler chickens.
添加外源蛋白酶的肉仔鸡饲粮中家禽副产物粕作为潜在蛋白质来源的研究
本试验旨在研究在家禽副产物粕配制的饲粮中添加外源蛋白酶对孵化后21 ~ 35 d肉鸡生长性能、小肠大小和肉理化性状的影响。试验选用120只1日龄“爱拔益加”肉仔鸡(公母比例为50:50),随机分为2种饲粮处理,每组6个重复,每笼10只鸡。2种饲粮处理包括对照饲粮(CON)和添加蛋白酶(CON+Pro)饲粮。第35天测定体重和采食量,计算整个试验期的饲料系数。每栏2只鸡实施安乐死,测量3段小肠的相对长度和相对重量,并在孵化后35天采集肉样品进行理化特征分析。添加外源蛋白酶对生长性能无影响(P >0.05),但有提高FCR的趋势(P =0.082)。添加蛋白酶有缩短小肠近端长度的趋势(P =0.091)。饲粮处理影响肉末颜色,添加蛋白酶降低肉末黄度比色值(P <0.001),显著降低肉末红度比色值(P =0.059)。由此可见,在禽副产物饲粮中添加外源蛋白酶对肉鸡生长性能、小肠大小和肉理化参数(CIE b *除外)均无影响。
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