The association of acetylacetate (Acr AB-Tol C) and QepA genes with multiple antibiotic resistance among Escherichia coli clinical isolates

Q3 Medicine

Reviews in Medical Microbiology Pub Date : 2020-07-01 DOI:10.1097/MRM.0000000000000181

Yaqdhan Alnomani, A. Ghasemian, M. Memariani, M. Eslami, Abdolreza Sabokrouh, A. F. Abbas, M. Shafiei

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引用次数: 0

Abstract

ISSN The role of efflux pumps in antibiotic resistance development among Escherichia coli may have been underappreciated. The objective of this study was assessment the association of AcrAB-TolCeffluxpumps and qepAgenes with resistance tocommonantibiotics among E. coli isolates. A total of 200 E. coli isolates were obtained from diverse samples of inpatients. Minimum inhibitory concentrations and Kirby–Bauer disk diffusion tests were determined for ceftazidime, cefotaxime, imipenem, gentamicin, and tetracycline. The Acr-AB-TolC and qepA genes were amplified using PCR technique and their association with antibiotics was also evaluated using Chi-square test. A majority of isolates (64%) were retrieved from gastrointestinal samples, followedby urinary tract infections (33%), and bloodstream (3%). All the isolates were resistant to ampicillin (100%), followed by cefazolin (59%), and cefoxitin (58%). However, 100% of the isolates showed susceptibility to fosfomycin. The prevalence of acrA, acrB, and qepA genes was 94% (n1⁄4188), 86% (n1⁄4172), and 8% (n1⁄416), respectively. The acrA and acrB were significantly associated with resistance to cefoxitin and cefazolin (P<0.01), ceftazidime (P<0.01), carbapenems (P1⁄40.022), and tetracycline (P1⁄40.0112). In addition, qepA gene was significantly associated with tetracycline resistance (P1⁄40.032). None of the patients had death outcome. A majority of E. coli isolates harbored the AcrAB genes, but qepA was observed among lower number of the isolates. It is notable that three strains lacked the extended spectrum beta-lactamase and carbapenemases and none of multidrug resistant strains carried tet and aminoglycoside modifying enzymes genes. Over-expression of efflux pumps has been increasingly is associated with clinically relevant antibiotic resistance. For this reason, the expression and functionality of efflux pumps should be more investigated profoundly and be compared between drug-resistant and drug-susceptible isolates. Copyright 2020 Wolters Kluwer Health, Inc. All rights reserved.

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乙酰乙酸(Acr AB-Tol C)和QepA基因与大肠杆菌临床分离株多重耐药的关系

外排泵在大肠杆菌抗生素耐药性发展中的作用可能被低估了。本研究的目的是评估acrab - tolcefflux泵和qepAgenes与大肠杆菌分离株对常见抗生素耐药性的关系。从住院病人的不同样本中共分离出200株大肠杆菌。测定头孢他啶、头孢噻肟、亚胺培南、庆大霉素和四环素的最低抑菌浓度和Kirby-Bauer盘片扩散试验。采用PCR技术扩增Acr-AB-TolC和qepA基因，并采用卡方检验评估其与抗生素的相关性。大多数分离株(64%)来自胃肠道样本，其次是尿路感染(33%)和血液(3%)。所有分离株均对氨苄西林耐药(100%)，其次是头孢唑林(59%)和头孢西丁(58%)。100%的菌株对磷霉素敏感。acrA、acrB和qepA基因的阳性率分别为94% (n1⁄4188)、86% (n1⁄4172)和8% (n1⁄416)。acrA和acrB与头孢西丁和头孢唑林耐药(P<0.01)、头孢他啶耐药(P<0.01)、碳青霉烯类耐药(P1⁄40.022)、四环素耐药(P1⁄40.0112)显著相关。此外，qepA基因与四环素耐药性显著相关(P1 / 40.032)。所有患者均无死亡结局。大多数大肠杆菌分离株含有AcrAB基因，但在少数分离株中观察到qepA基因。值得注意的是，3株菌株缺乏广谱β -内酰胺酶和碳青霉烯酶，多药耐药菌株均未携带tet和氨基糖苷修饰酶基因。外排泵的过度表达越来越多地与临床相关的抗生素耐药性相关。因此，应更深入地研究外排泵的表达和功能，并在耐药和药敏分离株之间进行比较。威科集团版权所有版权所有。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Reviews in Medical Microbiology 生物-微生物学

CiteScore

1.80

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Reviews in Medical Microbiology is a quarterly review journal which provides a balanced coverage of the whole field of medical microbiology. The Journal publishes state-of-the art reviews, mini-reviews, case presentations and original research from on-going research of the latest developments and techniques in medical microbiology, virology, mycology, parasitology, clinical microbiology, and hospital infection. In addition, PhD-Review - a platform for young researchers, and biographical Bio-Sketch articles are also considered. Reviews are concise, authoritative, and readable synthesis of the latest information on its subject, and references are limited to the fifty key sources for full reviews and twenty for mini-reviews. Reviews in Medical Microbiology is the perfect way for both qualified and trainee microbiologists, and researchers and clinicians with an interest in microbiology, to stay fully informed of the latest developments in medical microbiology. The journal is a valuable resource for educational and teaching purposes.