Genetic Polymorphism In Individuals With Type II Diabetes Mellitus Using PCR-RAPD In Sidoarjo District

Abstract

Received: December, 25, 2020 Revised: May, 4, 2021 Available online: May 2021 Diabetes Mellitus type II (T2DM) is a metabolic disorder. Its incidence increases from year to year. The control of T2DM incidence is problematic because it is involved genetic and environmental factors. Moreover, it can cause complications in people with infectious diseases. This study aims to determine the polymorphism of sufferers and non-sufferers of T2DM using the Polymerase Chain Reaction-Random Amplified Polymorphic DNA (PCR-RAPD) method. This research was descriptive-analytic with a cross-sectional approach. The sample consisted of 60 samples – 30 positive and 30 negative samples taken from several clinics in the Sidoarjo district. The primer used was A18 (5'AGGTGACCGT-3'). Data analysis used Chi-square with a 95% confidence level. The results produced 17 bands with the length of 197 bp, 239 bp, 269 bp, 319 bp, 390 bp, 530 bp, 588 bp, 686 bp, 777 bp, 972 bp, 1175 bp, 1676 bp, 2780 bp, 3843 bp, 6563 bp, 11072 bp & 18434 bp. The four bands were monomorphic. Two bands that showed significantly different results were 319bp (p=0.035) and 18434 bp (p=0.004). In conclusion, there are significant differences between people with and without T2DM in several fragments, namely 319 bp and 18434 bp bands. Further analysis needs to confirm the genes involved.