In vitro immunomodulatory, antifungal, and antibacterial screening of Phyllanthus niruri against to human pathogenic microorganisms

V. Shilpa, K. Muddukrishnaiah, B. Thavamani, V. Dhanapal, K. Arathi, K. Vinod, S. Sreeranjini
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引用次数: 11

Abstract

Background: Medicinal plants present a wide range of potentially phytochemical compounds that contain many useful properties including anticancer, enzyme inhibition, anti-inflammatory, antifungal, antibacterial, immunomodulatory, antioxidant, and antiallergic activities. Phyllanthus niruri capsules are extensively recommended to improve the function of the diseased liver. Its leaves root and the whole plant are used as an herbal complement. Aim: The present study was aimed to focus on the in vitro immunomodulatory activity, antifungal, antibacterial and phytochemical screening of aqueous, methanolic, and ethanolic extract of P. niruri. Materials and Methods: Immunomodulatory activities were evaluated through nitroblue tetrazolium assay. Antifungal and antibacterial activity were conducted against Candida albicans (NCIM - 3100), Aspergillus niger (NCIM - 1028), Eschericha coli (NCIM - 5346), Bacillus subtilis (NCIM - 2920), and Staphylococcus aureus (NCIM - 5345) by using disc diffusion method. Results: Medicinal plants contain polyphenolic compounds which have potent anti-cancer and immunomodulator activity. P. niruri has potential immunomodulatory activity. Aqueous, methanolic, and ethanolic extract of P. niruri did not show any significant antifungal activity and 100 mg/ml, 150 mg/ml, and 200 mg/ml. Aqueous, methanolic, and ethanolic extract showed significant antibacterial activity. Conclusion: From this study, it is concluded that P. niruri does not have antifungal activity but has potent immunomodulatory and antibacterial activity. This immunomodulatory and antibacterial activity of P. niruri may be due to the secondary metabolites such as alkaloid, tannins, terpenoids, flavonoids, and phenol compounds.
余甘子对人致病性微生物的体外免疫调节、抗真菌和抗菌筛选
背景:药用植物具有广泛的潜在植物化学成分,含有许多有用的特性,包括抗癌、酶抑制、抗炎、抗真菌、抗菌、免疫调节、抗氧化和抗过敏活性。余甘子胶囊被广泛推荐用于改善病变肝脏的功能。它的叶子、根和整个植物被用作草药补充。目的:研究水合、甲醇和乙醇提取物的体外免疫调节活性、抗真菌、抗菌和植物化学筛选。材料与方法:采用硝基蓝四氮唑法测定其免疫调节活性。采用盘片扩散法对白色念珠菌(NCIM - 3100)、黑曲霉(NCIM - 1028)、大肠杆菌(NCIM - 5346)、枯草芽孢杆菌(NCIM - 2920)和金黄色葡萄球菌(NCIM - 5345)进行抑菌和抑菌活性测定。结果:药用植物中含有较强的抗癌和免疫调节活性的多酚类化合物。P. niruri具有潜在的免疫调节活性。在100mg /ml、150mg /ml和200mg /ml的水提液、甲醇提液和乙醇提液中均未表现出显著的抗真菌活性。水提物、甲醇提物和乙醇提物具有显著的抗菌活性。结论:该菌不具有抗真菌活性,但具有较强的免疫调节和抗菌活性。这种免疫调节和抗菌活性可能是由于其次生代谢产物,如生物碱、单宁、萜类、黄酮类和酚类化合物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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