Expressions of MMP2, TIMP2, Ki-67 and P53 in glioma tissues and their significance

Abstract

Objective To investigate the expressions and significance of matrix metalloproteinase 2 (MMP2), tissue inhibitor of metalloproteinase 2 (TIMP2), Ki-67 and P53 in human glioma tissues. Methods The expressions of Ki-67 and P53 in paraffin samples of 50 gliomas (immunohistochemistry SP method) from January 1995 to December 2015 in Qilu Hospital of Shandong University were analyzed retrospectively, and the expressions of MMP2 and TIMP2 were detected by immunohistochemistry. The differences of parameters between high- and low-grade gliomas were compared by χ2 test and their correlations were assessed by Spearman correlation analysis. Results In the high-grade group (grade Ⅲ-Ⅳ, n=37), the high expression rate of MMP2 was 81.08% (30/37), the positive expression rates of Ki-67 and P53 were 78.38% (29/37) and 72.97% (27/37). In the low-grade group (grade Ⅰ-Ⅱ, n=13), there were 2 patients with high expression of MMP2, 3 patients with positive expression of Ki-67 and P53 respectively, and there were significant differences between the two groups (χ2=15.282, P<0.001; χ2=10.482, P=0.001; χ2=9.979, P=0.002). A significant correlation was found between them and pathological grade (r=0.600, P<0.001; r=0.505, P<0.001; r=0.447, P=0.001). The high expression of TIMP2 was found in 8 cases of low-grade group and 19 cases (51.35%) of high-grade group, with no significant difference (χ2=0.402, P=0.526). The expression of MMP2 was positively correlated with Ki-67 and P53 (r=0.392, P=0.005; r=0.323, P=0.022), while TIMP2 was negatively correlated with Ki-67 (r=-0.441, P=0.001). The expression of P53 was positively correlated with Ki-67 (r=0.748, P<0.001). Conclusion MMP2, Ki-67 and P53 may play important role in the proliferation and invasiveness of glioma. The mechanism of TIMP2 is complicated and needs further study. Key words: Glioma; Matrix metalloproteinase 2; Tissue inhibitor of metalloproteinase 2; Ki-67 antigen; Tumor suppressor protein P53