Antimicrobial Resistance in Escherichia coli: a Cross Sectional Study in Chicken Poultry of Kirtipur, Nepal

Manisha Kharel, Sumi Tamaru, Tirtha Raj Chaudary, Dabin Thapa Magar, Bishow Raj Gaire, Bindu Ghimire
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引用次数: 1

Abstract

Objectives: The study was conducted to determine antimicrobial susceptibility pattern and prevalence of Extended Spectrum Beta Lactamase (ESBL) producing E. coli from fecal sample of different chicken poultry farm located at Kirtipur. Methods: The cross sectional study was conducted from November 1st 2019 to February 29th 2020. The samples were collected from 27 different poultry farms and processed at Microbiology laboratory at Trichandra Multiple Campus. Identified E. coli were subjected to antimicrobial susceptibility test by using Kirby and Bauer Disc Diffusion technique and Combined disk method was used to determine ESBL E. coli. Results: From all 27 poultry farms, E. coli was isolated from broiler (n=13), layer (n=10), and local (n=4) breeds, of which 23 (85.18%) were not registered. The chickens were fed with vitamin and calcium as growth promoter along with antibiotics; Piperacillin, Colistin and Doxycycline. Altogether 85.18% (n=23) isolates showed sensitivity towards Nitrofurantoin, Cefotaxime and Ceftazidime followed by Tigecycline 77.7% (n=21). Among these isolates 66.6% (n=18) were resistance towards Piperacillin followed by Ampicillin 37% (n=10). A statistically significant correlation was seen in resistance rate between broiler and layers. Among total isolates 37.03% (n=10) were Multi Drug Resistance (MDR) and 14.81% (n=4) were ESBL producer. Unregistered poultry farms were associated with MDR and ESBL Ec isolates. Conclusion: Unregistered poultry farms and irrational use of antibiotics has influenced development of MDR and ESBL isolates. Timely monitoring and surveillance is suggested to decrease the trend of antimicrobial resistance (AMR) in poultry system.
大肠杆菌的抗菌素耐药性:尼泊尔基尔蒂普尔鸡禽的横断面研究
目的:研究克尔蒂普尔不同鸡禽养殖场粪便样品中产生扩展谱β -内酰胺酶(ESBL)的大肠杆菌的药敏模式和流行情况。方法:横断面研究于2019年11月1日至2020年2月29日进行。样本从27个不同的家禽养殖场采集,并在Trichandra多校区微生物实验室进行处理。鉴定出的大肠杆菌采用Kirby和Bauer纸片扩散法进行药敏试验,联合纸片法检测ESBL大肠杆菌。结果:27个家禽养殖场分别从肉鸡(n=13)、蛋鸡(n=10)和地方(n=4)种中分离出大肠杆菌,其中23种(85.18%)未登记。在添加抗生素的同时,添加维生素和钙作为生长促进剂;哌拉西林,粘菌素和强力霉素。共有85.18% (n=23)的菌株对呋喃妥因、头孢噻肟和头孢他啶敏感,其次是替加环素77.7% (n=21)。对哌拉西林耐药66.6% (n=18),氨苄西林耐药37% (n=10)。肉鸡与蛋鸡耐药率呈显著相关。其中耐多药菌株(MDR)占37.03% (n=10),产生ESBL菌株占14.81% (n=4)。未登记的家禽养殖场与耐多药和ESBL Ec分离株有关。结论:未经注册的家禽养殖场和不合理的抗生素使用影响了耐多药和ESBL分离株的发展。建议及时进行监测和监测,以降低家禽系统中抗菌素耐药性的趋势。
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