Impact of chronic irradiation of IMV 9096 and IMV 8614 strains of Pseudomonas aeruginosa on immunomodulatory properties of their lipopolysaccharide complex

IF 0.4 Q4 PHYSICS, NUCLEAR

Nuclear Physics and Atomic Energy Pub Date : 2021-12-25 DOI:10.15407/jnpae2021.04.375

J. V. Shylina, O. Molozhava, S. Litvinov, O. P. Dmitriev

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引用次数: 0

Abstract

The effect of chronic irradiation of Pseudomonas aeruginosa (P. aeruginosa) phytopathogenic strain IMV 9096 and saprophytic strain IMV 8614 at a dose rate of 0.19 µGy/s of γ-radiation in the dose field of 137Cs on the immunomodulatory properties of their lipopolysaccharide (LPS) has been investigated. It was shown that in the wild-type seedlings of Arabidopsis thaliana (A. thaliana) Col-0 pre-treatment with LPS 9096, isolated from both irradiated and non-irradiated bacterial culture, caused an increased harmful effect 2.8 - 5.6 fold when plants were then infected with bacteria of this strain. Seedling damage was more pronounced with the use of LPS, isolated from bacteria exposed to chronic radiation. In seedlings of mutant jin1 with impaired jasmonate signaling pre-treatment of LPS 9096 caused attenuation of the damage at 20 - 45 % when infected with P. aeruginosa 9096. Pre-treatment of Arabidopsis seeds with bacterial LPS 8614, obtained from both irradiated and non-irradiated P. aeruginosa 8614 cultures, had a non-significant effect (± 15 % over control). In mutant plants, jin1 pre-treatment of seeds with LPS 8614 led to increased damage when infected with P. aeruginosa IMV 9096 at 30 - 60 %. It was found that chronic irradiation of bacteria changes the immunomodulatory properties of their LPS and the effect depends on the bacterial strain. This effect is mediated by jasmonate and salicylate signaling systems.

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慢性照射铜绿假单胞菌IMV 9096和IMV 8614菌株对其脂多糖复合物免疫调节特性的影响

研究了在137Cs剂量场下，以0.19 μ Gy/s的γ辐射速率慢性照射铜绿假单胞菌(P. aeruginosa)植物致病菌株IMV 9096和腐生菌株IMV 8614对其脂多糖(LPS)免疫调节特性的影响。结果表明，在拟南芥(A. thaliana) col0野生型幼苗中，经辐照和未辐照细菌培养分离的LPS 9096预处理后，该菌株的有害效应增加了2.8 ~ 5.6倍。使用从暴露于慢性辐射的细菌中分离出来的LPS对幼苗的损害更为明显。在茉莉酸信号通路受损的突变株jin1的幼苗中，LPS 9096处理后，感染P. aeruginosa 9096后，损伤减弱了20 - 45%。从辐照和未辐照的P. aeruginosa 8614培养物中获得的细菌LPS 8614预处理拟南芥种子，效果不显著(比对照组±15%)。在突变株中，用LPS 8614预处理jin1种子，当铜绿假单胞菌IMV 9096感染30 ~ 60%时，损伤增加。研究发现，长期照射细菌会改变其脂多糖的免疫调节特性，其影响与菌株有关。这种作用是由茉莉酸盐和水杨酸盐信号系统介导的。

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来源期刊

Nuclear Physics and Atomic Energy PHYSICS, NUCLEAR-

CiteScore

0.70

自引率

33.30%

发文量

审稿时长

19 weeks

期刊介绍： The journal Nuclear Physics and Atomic Energy presents the publications on Nuclear Physics, Atomic Energy, Radiation Physics, Radioecology, Engineering and Methods of Experiment. The journal includes peer-reviewed articles which are completed works containing new results of theoretical and experimental researches and are of interest for the scientists, postgraduate students, engineers and for the senior students.