SSR marker based profiling and diversity analysis of mungbean [Vigna radiata (L.) Wilczek] genotypes

Abstract

The evaluation and characterization of germplasm of mungbean are considered to be essential prerequisites for a rational use of its genetic resources. Accordingly in the present study, genetic diversity in 18 mungbean genotypes was assessed using a panel of 40 microsatellite based primer pairs. Out of 40 primers initially tested, only 24 showed distinct polymorphism and consequently only these primers were utilized for the purpose of genome profiling of the entries. Altogether, 183 allelic variants were detected with an average of 4.95 polymorphic fragment per primer. The number of amplified loci varied from 4 (CEDG 15) to a maximum of 12 (CEDG 92 and CEDG 172) with an average of 4.95 polymorphic fragment per primer. The polymorphic information content (PIC) ranged from 0.440 to 0.928 with an average of 0.822 per SSR primer pair. The lowest polymorphism percent was observed for primer CEDGAG001 (16.67) while the primer CEDG 154 gave the maximum polymorphism percent (63.64). The primer pairs CEDG 008, CEDG 068 and CEDG 154 among the total primer pairs were found to be highly informative. Dice's similarity coefficient ranged from 0.03 to 0.58. Dendrogram based on SSR data grouped the mungbean genotypes into seven clusters. The pattern of clustering was also reiterated by the results of principal component analysis (PCA). The SSR primer based analysis allowed unique and unambiguous genotyping of the entries. The genetic diversity observed in the present study could be useful to know the phylogenetic links among the cultivars and for the selection of suitable parents to be further used in breeding programmes and genetic mapping studies.