Genetic analysis of Toxocara cati (Nematoda: Ascarididae) from Guangdong province, subtropical China

IF 1.1 4区生物学 Q4 GENETICS & HEREDITY

Mitochondrial Dna Part a Pub Date : 2018-01-02 DOI:10.1080/24701394.2016.1258404

Xi He, Meng-Na Lv, Guo-Hua Liu, R. Lin

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引用次数: 15

Abstract

Abstract Toxocara cati (cat roundworm) is a common parasitic nematode that infects humans and other hosts, causing toxocariasis. Although its significance as a pathogen, the epidemiology, genetics and biology of T. cati remain poorly understand in China. In the present study, genetic variation in mitochondrial (mt) cytochrome c oxidase subunit 1 (cox1) gene and internal transcribed spacer (ITS) of rDNA region among T. cati in Guangdong province, subtropical China was examined. A portion of the cox1 (pcox1) and the complete ITS (ITS1 + 5.8S rDNA + ITS2) were amplified separately from individual worms by polymerase chain reaction (PCR) and amplicons were then subjected to sequencing from both directions. The length of the sequences of pcox1, ITS-1, and ITS-2 were 308 bp, 462 bp, and 335 bp, respectively. The intra-specific sequence variations within T. cati were 0–3.6% for pcox1, 0–2.4% for ITS-1, and 0–2.7% for ITS-2. However, the inter-specific sequence differences were significantly higher, being 8.6%, 10.7%, and 11.3% for pcox1, ITS-1, and ITS-2, respectively. Phylogenetic analyses based on the pcox1 sequences indicated that all the isolates in Guangdong province were in genus Toxocara, which confirmed that these parasites represent T. cati. The molecular approach employed provides a powerful tool for elucidating the epidemiology, genetics, and biology of zoonotic T. cati in China and elsewhere.

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标题广东省猫弓形虫遗传分析(线虫纲:蛔虫科)

猫蛔虫(Toxocara cati)是一种常见的寄生线虫，可感染人类和其他宿主，引起弓形虫病。虽然其作为一种病原的重要性，但其流行病学、遗传学和生物学在中国仍知之甚少。研究了广东地区猫的线粒体(mt)细胞色素c氧化酶亚基1 (cox1)基因和rDNA区域内转录间隔区(ITS)的遗传变异。利用聚合酶链反应(PCR)分别从虫体中扩增出部分cox1 (pcox1)和完整ITS (ITS1 + 5.8S rDNA + ITS2)，然后从两个方向对扩增子进行测序。pcox1、ITS-1和ITS-2的序列长度分别为308 bp、462 bp和335 bp。猫绦虫的pco1、ITS-1和ITS-2的特异性序列变异分别为0-3.6%、0-2.4%和0-2.7%。然而，pcox1、ITS-1和ITS-2的种间序列差异明显更高，分别为8.6%、10.7%和11.3%。基于pcox1序列的系统发育分析表明，广东省分离的弓形虫均为弓形虫属，证实该寄生虫为猫绦虫。所采用的分子方法为阐明中国和其他地区人畜共患猫绦虫的流行病学、遗传学和生物学提供了有力的工具。

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来源期刊

Mitochondrial Dna Part a Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.00

自引率

0.00%

发文量

期刊介绍： Mitochondrial DNA Part A publishes original high-quality manuscripts on physical, chemical, and biochemical aspects of mtDNA and proteins involved in mtDNA metabolism, and/or interactions. Manuscripts on cytosolic and extracellular mtDNA, and on dysfunction caused by alterations in mtDNA integrity as well as methodological papers detailing novel approaches for mtDNA manipulation in vitro and in vivo are welcome. Descriptive papers on DNA sequences from mitochondrial genomes, and also analytical papers in the areas of population genetics, phylogenetics and human evolution that use mitochondrial DNA as a source of evidence for studies will be considered for publication. The Journal also considers manuscripts that examine population genetic and systematic theory that specifically address the use of mitochondrial DNA sequences, as well as papers that discuss the utility of mitochondrial DNA information in medical studies and in human evolutionary biology.

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