Effect of Deferoxamine on Rat Fetal Bone Formation and on it is Certain Regulating Genes

Abstract

The aim of this study is to investigate the deferoxamine effect on rat fetal bone formation and on certain regulating genes expression that play a vital key role in bone formation. Sixty albino adult female rats three months old with average weight 170-200g were employed in this study. The rats were distributed randomly in to two groups. Control group (n=30) receiving 0.3ml ip physiological saline and deferoxamine methane intra peritoneal daily at a dose of 100 mg/kg throughout the pregnancy from the first day of pregnancy until last day of pregnancy. Skeleton samples and staining were done on sixty fetuses were chosen from each group at 20 days of pregnancy as well as liver samples from mothers and their fetuses for studying gene expression of Msx1 and Cx43. The results showed that many variations in certain bone formation and development markers were recorded as well as there was downregulation of studied genes. In conclusion, the study found that deferoxamine affect negatively on certain bone development markers and on gene expression of Msx1 and Cx43 represented as downregulation.