{"title":"Acetohydroxyacid Synthase from Cell Suspension Cultures of Isatis tinctoria l. and Ruta graveolens l.","authors":"Walter Maier , Brigitte Schumann , Detlef Gröger","doi":"10.1016/S0015-3796(11)80003-X","DOIUrl":null,"url":null,"abstract":"<div><p>Acetohydroxyacid synthase (EC 4.1.3.18) has been extracted from cell suspension cultures of <em>Isatis tinctoria</em> (Cruciferae) and <em>Ruta graveolens</em> (Rutaceae). A combination of salt precipitation, gel filtration and ion exchange chromatography was used for partial purification. The apparent molecular masses of AHAS were M<sub>r</sub> 82,000 and 85,000 for <em>Isatis</em> and <em>Ruta</em>, respectively. FAD was an absolute requirement for AHAS activity. The apparent K<sub>m</sub> values of <em>Isatis</em>-AHAS are the following ones: FAD 6,3 × 10<sup>−6</sup> M; TPP 6,3 × 10<sup>−6</sup> M; pyruvate 7 × 10<sup>−3</sup>, and 6 × 10<sup>−3</sup> M (for <em>Ruta</em>-AHAS).</p><p>Branched-chain amino acids and chlorsulfuron are feedback inhibitors for <em>Isatis</em>-AHAS but acetohydroxyacid synthase from <em>Ruta</em> is not sensitive to valine, leucine and isoleucine.</p></div>","PeriodicalId":8798,"journal":{"name":"Biochemie und Physiologie der Pflanzen","volume":"188 3","pages":"Pages 161-167"},"PeriodicalIF":0.0000,"publicationDate":"1992-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0015-3796(11)80003-X","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemie und Physiologie der Pflanzen","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S001537961180003X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
Abstract
Acetohydroxyacid synthase (EC 4.1.3.18) has been extracted from cell suspension cultures of Isatis tinctoria (Cruciferae) and Ruta graveolens (Rutaceae). A combination of salt precipitation, gel filtration and ion exchange chromatography was used for partial purification. The apparent molecular masses of AHAS were Mr 82,000 and 85,000 for Isatis and Ruta, respectively. FAD was an absolute requirement for AHAS activity. The apparent Km values of Isatis-AHAS are the following ones: FAD 6,3 × 10−6 M; TPP 6,3 × 10−6 M; pyruvate 7 × 10−3, and 6 × 10−3 M (for Ruta-AHAS).
Branched-chain amino acids and chlorsulfuron are feedback inhibitors for Isatis-AHAS but acetohydroxyacid synthase from Ruta is not sensitive to valine, leucine and isoleucine.
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