Validation of a method employing liquid chromatography to identify and quantify glycomacropeptide of casein in raw milk

Q3 Pharmacology, Toxicology and Pharmaceutics

Vitae Pub Date : 2021-08-11 DOI:10.17533/udea.vitae.v28n3a345256

Orlando Bernal Chia, Fernando Murcia Rubiano, Olga Lucía Ávila Guzmán, Jenifer Yaneth Guzman Gualteros, Willinton Arturo Arias Casallas, Maria Victoria Morales

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引用次数: 1

Abstract

BACKGROUND: The information on official testing methods, or regulatory methods in Colombia to test whey in milk is limited; this restriction of information goes against the possibility of mitigating the risk of food fraud. OBJECTIVES: The validation of an HPLC method to determine casein glycomacropeptide (c-GMP), a protein that countries such as Brazil, Spain, and Ecuador have used as an indicator of raw milk adulteration with whey, was carried out. METHODS: A 10mL sample of raw milk is precipitated with 24% TCA using ultrasound, a process followed by filtration. The collected fraction ensured the separation of c-GMP and then injected into the liquid chromatography. RESULTS: A 30 minutes analysis allowed the determination of c-GMP with a retention time of 12.9 ± 0.5 minutes. The performance characteristics method in the validation exercise were: recovery percentage 99.97%, linearity R2> 0.95; % RSD accuracy <5.3%. CONCLUSION, the method exhibits desirable attributes for the intended purpose.

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用液相色谱法鉴定和定量原料奶中酪蛋白糖宏肽的方法的验证

背景:哥伦比亚官方检测方法或监管方法检测牛奶中乳清的信息有限;这种对信息的限制不利于减轻食品欺诈风险的可能性。目的:建立了一种高效液相色谱法测定酪蛋白糖宏肽(c-GMP)的方法，该蛋白质被巴西、西班牙和厄瓜多尔等国用作原料奶掺入乳清的指标。方法:取10mL原料乳样品，用24% TCA超声沉淀，然后过滤。收集的部分保证了c-GMP的分离，然后进入液相色谱。结果:c-GMP检测时间为30分钟，保留时间为12.9±0.5分钟。本方法的性能特征为:回收率99.97%，线性R2> 0.95;% RSD准确度<5.3%。结论:该方法显示了预期目的的理想属性。

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来源期刊

Vitae PHARMACOLOGY & PHARMACY-

CiteScore

1.20

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： The journal VITAE is the four-monthly official publication of the School of Pharmaceutical and Food Sciences, and its mission is the diffusion of the scientific and investigative knowledge in the various fields of pharmaceutical and food research, and their related industries. The Journal VITAE is an open-access journal that publishes original and unpublished manuscripts, which are selected by the Editorial Board and then peer-reviewed. The editorial pages express the opinion of the Faculty regarding the various topics of interest. The judgments, opinions, and points of view expressed in the published articles are the responsibility of their authors.