2,2’-Diphenyl-1-picrylhydrazyl and 2,2′-azinobis-(3-ethyl-benzothiazoline-6-sulfonic acid) scavenging assay of Extract and Fractions of Rambutan (Nephelium Lappaceum L.) Seed

M. Budikafa, Rumiyati, S. Riyanto, A. Rohman
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Abstract

This research was intended to evaluate the antiradical activity of extracts and fractions of rambutan seed using 2,2’- diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+). The powder of rambutan seed was subjected to maceration with methanol. The methanolic extract obtained was then fractionated using petroleum ether (PE), dichloromethane (DCM) and ethyl acetate (EA) to obtain PE, DCM and EA fractions, respectively. The methanol extract and fractions were then assayed for antiradical activities and phenolics and flavonoid contents.The results showed that EA fraction has lowest IC50 values among extract and fractions evaluated, i.e. 244.6 ± 2.1 μg/ml using DPPH radical. Using ABTS radical, EA fraction (0.1%) also revealed the highest antiradical activity of 643.4 ± 34.9 μM trolox equivalent. However, these antiradical activities were lower than those in positive controls used (vitamin C and vitamin E). The antiradical activities of extract and fractions correlated with total phenolics and flavonoid contents. The seed of rambutan fruit could be exploited as natural antioxidants to be used in food supplements. Dhaka Univ. J. Pharm. Sci. 18(2): 145-152, 2019 (December)
红毛丹(Nephelium Lappaceum L.)提取物和馏分的2,2 ' -二苯基-1-吡啶肼和2,2 ' -氮化萘-(3-乙基-苯并噻唑-6-磺酸)清除率测定种子
本研究采用2,2′-二苯基-1-吡啶肼(DPPH)和2,2′-氮唑-(3-乙基苯并噻唑-6-磺酸)(ABTS•+)对红毛丹种子提取物和组分的抗自由基活性进行了评价。对红毛丹种子粉末进行甲醇浸渍。将得到的甲醇提取物分别用石油醚(PE)、二氯甲烷(DCM)和乙酸乙酯(EA)进行分馏,得到PE、DCM和EA馏分。然后测定甲醇提取物和馏分的抗自由基活性、酚类物质和类黄酮含量。结果表明,在DPPH自由基作用下,EA部位的IC50值最低,为244.6±2.1 μg/ml。使用ABTS自由基时,EA组分(0.1%)的抗自由基活性最高,为643.4±34.9 μM trolox当量。但其抗自由基活性低于阳性对照(维生素C和维生素E),其抗自由基活性与总酚类和类黄酮含量呈正相关。红毛丹种子可作为天然抗氧化剂开发利用于食品补充剂中。达卡大学药学院。科学通报,18(2):145-152,2019 (12)
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