MICROPROPAGATION OF ACTINIDIA THROUGH IN VITRO CULTURE

Abstract

In an effort to elaborate the elements of the actinidia micropropagation methodology, 2 varieties of the male form (Kamandor, Pryvabny) and the female form (Sentyabrskaya, Prevoshodnaya) were selected. The shoot tips of the male forms and buds of lignified shoots of the male and female forms were used as initial explants. The regenerative ability of actinidia explants at the stage of in vitro culture induction was defined by the plant genotype and the type of explant selected: explants of male forms of actinidia (Pryvabny and Kamandor varieties) were characterized by high viability when using shoot tips – 66.66–100 %, vegetative buds – 16.66–80 %. At the same time for female forms – 10–22.73 %. The sterilization scheme was chosen. A long period of stabilization in the first passages, the absence of shoot initiation and their growth were noted. The reproduction factor for male forms on average over passages was 2.7, while for female forms it was 1.4. The replacement of the carbohydrate source and the change in the composition of growth regulators made it possible to increase the reproduction rate in male forms by one and a half times.