Use of Leuconostoc Mesenteroides to Produce a Dextran Bioflocculant

Q3 Environmental Science

Environmental Research, Engineering and Management Pub Date : 2022-04-01 DOI:10.5755/j01.erem.78.1.29591

M. De La Cruz-Noriega, S. Rojas-Flores, Santiago M. Benites, M. A. Quezada Álvarez, N. M. Otiniano García, Magda Rodríguez Yupanqui

{"title":"Use of Leuconostoc Mesenteroides to Produce a Dextran Bioflocculant","authors":"M. De La Cruz-Noriega, S. Rojas-Flores, Santiago M. Benites, M. A. Quezada Álvarez, N. M. Otiniano García, Magda Rodríguez Yupanqui","doi":"10.5755/j01.erem.78.1.29591","DOIUrl":null,"url":null,"abstract":"In this study, we aimed to determine the in vitro activity of Leuconostoc mesenteroides var. mesenteroides isolatedfrom sugar-industry effluents to produce a dextran bioflocculant from sucrose as a low-cost substrate.L. mesenteroides strains present in residual cane juice from a sugar factory were isolated and biochemicallyidentified using Mayeux, Sandine, and Elliker agar (MSE) as a selective medium. The strain number 3 (LM03) wasbiochemically identified as L. mesenteroides var. mesenteroides, which was used for this study. The concentrationof dextran was quantified by dry weight, the morphology and purity were evaluated using Fourier-transforminfrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy(EDS). Flocculation was evaluated via turbidimetric assays in different pH ranges from sugar-industry effluentsand doses of dextran.To evaluate the flocculant activity according to the effect of pH, a jar test kit from Phipps and Bird, USA, wasused with the sample recollected from the effluent (sugar industry). The pH of the samples was adjusted to 7, 8,9, 10 and 11, with a dose of 40 ppm (dextran dose) at a fast and slow speed of 150 and 50 rpm, respectively. Toevaluate the influence of the dose of dextran, values of 5, 20 and 40 ppm were used with fast speeds of 180–150rpm and slow speeds of 30–50 rpm, respectively.The strain (LM03) was able to produce the highest concentration of dextran (26.87 g/L) in 76 h of incubation. Thepresence of dextran was identified in the MSE agar after incubation and characterized by FTIR, SEM, and EDS.Besides that, we observed that the best flocculation activity was observed at a pH of 9 and a concentration of 40ppm of dextran, with a fast agitation speed of 150 rpm for 5 min and a slow agitation speed of 50 rpm for 15 min,achieving 77.7% removal of turbidity from the sugar factory effluent.L. mesenteroides was responsible for the bioflocculation of dextran in different sugar-industry effluents","PeriodicalId":11703,"journal":{"name":"Environmental Research, Engineering and Management","volume":"82 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental Research, Engineering and Management","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5755/j01.erem.78.1.29591","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Environmental Science","Score":null,"Total":0}

引用次数: 2

Abstract

In this study, we aimed to determine the in vitro activity of Leuconostoc mesenteroides var. mesenteroides isolatedfrom sugar-industry effluents to produce a dextran bioflocculant from sucrose as a low-cost substrate.L. mesenteroides strains present in residual cane juice from a sugar factory were isolated and biochemicallyidentified using Mayeux, Sandine, and Elliker agar (MSE) as a selective medium. The strain number 3 (LM03) wasbiochemically identified as L. mesenteroides var. mesenteroides, which was used for this study. The concentrationof dextran was quantified by dry weight, the morphology and purity were evaluated using Fourier-transforminfrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy(EDS). Flocculation was evaluated via turbidimetric assays in different pH ranges from sugar-industry effluentsand doses of dextran.To evaluate the flocculant activity according to the effect of pH, a jar test kit from Phipps and Bird, USA, wasused with the sample recollected from the effluent (sugar industry). The pH of the samples was adjusted to 7, 8,9, 10 and 11, with a dose of 40 ppm (dextran dose) at a fast and slow speed of 150 and 50 rpm, respectively. Toevaluate the influence of the dose of dextran, values of 5, 20 and 40 ppm were used with fast speeds of 180–150rpm and slow speeds of 30–50 rpm, respectively.The strain (LM03) was able to produce the highest concentration of dextran (26.87 g/L) in 76 h of incubation. Thepresence of dextran was identified in the MSE agar after incubation and characterized by FTIR, SEM, and EDS.Besides that, we observed that the best flocculation activity was observed at a pH of 9 and a concentration of 40ppm of dextran, with a fast agitation speed of 150 rpm for 5 min and a slow agitation speed of 50 rpm for 15 min,achieving 77.7% removal of turbidity from the sugar factory effluent.L. mesenteroides was responsible for the bioflocculation of dextran in different sugar-industry effluents

查看原文本刊更多论文

利用肠系膜白菌生产右旋糖酐生物絮凝剂

在这项研究中，我们旨在确定从制糖工业废水中分离的肠系膜芽孢杆菌(Leuconostoc mesenteroides)的体外活性，以蔗糖作为低成本底物生产葡聚糖生物絮凝剂。采用Mayeux、Sandine和Elliker琼脂(MSE)作为选择培养基，对某糖厂残蔗汁中的肠系膜菌株进行了分离和生化鉴定。菌株3号(LM03)经生化鉴定为L. mesenteroides var. mesenteroides，用于本研究。用干重法测定葡聚糖的浓度，用傅里叶变换红外光谱(FTIR)、扫描电镜(SEM)和能量色散x射线光谱(EDS)评价葡聚糖的形貌和纯度。通过浊度法测定制糖工业废水在不同pH范围和葡聚糖剂量下的絮凝效果。为了根据pH值的影响来评估絮凝剂的活性，使用了美国Phipps and Bird公司的罐子测试试剂盒，对从废水(制糖工业)中收集的样品进行了测试。将样品的pH调整为7、8、9、10和11，以40 ppm(葡聚糖剂量)的剂量分别在150和50 rpm的快、慢转速下进行。为了评估右旋糖酐剂量的影响，分别在180-150rpm的快转速和30 - 50rpm的慢转速下使用5,20和40ppm的值。菌株LM03在培养76 h后葡聚糖的浓度最高，为26.87 g/L。培养后在MSE琼脂中发现葡聚糖的存在，并通过FTIR, SEM和EDS进行了表征。此外，我们还观察到，在pH = 9、葡聚糖浓度为40ppm的条件下，快速搅拌速度为150转/分，搅拌5分钟，慢速搅拌速度为50转/分，搅拌15分钟，絮凝活性最佳，糖厂出水浊度去除率达到77.7%。肠系膜菌负责葡聚糖在不同制糖工业废水中的生物絮凝

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Environmental Research, Engineering and Management Environmental Science-Environmental Engineering

CiteScore

2.40

自引率

0.00%

发文量

期刊介绍： First published in 1995, the journal Environmental Research, Engineering and Management (EREM) is an international multidisciplinary journal designed to serve as a roadmap for understanding complex issues and debates of sustainable development. EREM publishes peer-reviewed scientific papers which cover research in the fields of environmental science, engineering (pollution prevention, resource efficiency), management, energy (renewables), agricultural and biological sciences, and social sciences. EREM’s topics of interest include, but are not limited to, the following: environmental research, ecological monitoring, and climate change; environmental pollution – impact assessment, mitigation, and prevention; environmental engineering, sustainable production, and eco innovations; environmental management, strategy, standards, social responsibility; environmental economics, policy, and law; sustainable consumption and education.