Development of SCAR Molecular Markers in Eucalyptus saligna and Eucalyptus tereticornis

Abstract

The genus Eucalyptus includes over 700 species, some of which are the most widely planted hardwoods worldwide. Each species of Eucalyptus present different characteristics regarding its wood quality and yield. This fact makes it very important to work with known species to optimize handling and conservation of forest resources. Some of them are morphologically similar, making it difficult to differentiate by simple observation. An alternative approach is to develop molecular methods for the species differentiation. Using a Bulk Segregant Analysis (BSA) with 59 RAPD (Random-Amplified Polymorphic DNA) primers of Operon Technologies Inc. Kits, polymorphic DNA fragments between Eucalyptus species were isolated and SCAR (Sequence Characterized Amplified Regions) markers designed for Eucalyptus saligna and Eucalyptus tereticornis.