Effect of fluoride on expression of osteoblast Runx2, and Osterix and COL I A2

Q3 Medicine

中华地方病学杂志 Pub Date : 2011-01-20 DOI:10.3760/CMA.J.ISSN.1000-4955.2011.01.007

Zhang Ya-lou, Liu Kai-tai, Liu Ji-wen, Zhong Jin-jie

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Abstract

Objective To study the effect of fluoride on expression of osteoblast Runx2, Osterix and their downstream COL I A2 in vitro. Methods Human osteoblast Saos-2 was cultured in vitro. The cells were grouped according to fluoride(NaF) dose used: 0(control ), 0.625,1.250,2.500,5.000,10.000,20.000,40.000,80.000,160.000 mg/L. Cells were collected after 24 h culture, RNA extracted, and the mRNA expression of Runx2 and Osterix and downstream genes COL I A2 was detected using fluorescent quantitative reverse transcription polymerase chain reaction [Real-time (RT)-PCR]). Results After 24 h in vitro cell cultivation with NaF, the expression of Runx2 in 0.625,1.250,2.500,5.000,10.000,20.000 mg/L groups(388.00 ± 41.80,209.00 ± 25.80,42.80 ±4.52,63.00 ± 16.10,24.30 ± 4.23,16.20 ± 4.32) was higher than that of the control group( 1.00 ± 0.12, all P ＜0.05). The expression of Runx2 in 40.000,80.000,160.000 mg/L groups(0.40 ± 0.05,1.91 ± 0.28,4.87±1.36)compared with that of control group, the difference was statistically insignificant(all P ＞ 0.05).The expression of Osterix mRNA in 1.250,2.500,5.000 mg/L groups(4.04 ± 1.67,229.00 ± 51.00,46.40 ± 10.60) was higher than that of the control group( 1.00 ± 0.42,all P ＜ 0.05). The expression of Osterix mRNA in 10.000,20.000,40.000,80.000,160.000 mg/L groups(0. 16 ± 0.07,0.13 ± 0.01,1.73 ± 0.54,0.01 ± 0.01, 0.09 ± 0.01) compared with that of control group, the difference was statistically insignificant (all P ＞ 0.05). The expression of COL I A2 mRNA in 0.625,1.250,2.500,5.000,10.000,20.000 mg/L groups (2.27 ± 0.89,8.03 ± 2.31,14.20 ± 2.75,7.66 ± 1.34,8.96 ±2.30) was higher than that of the control group (1.00 ± 0.04, all P ＜ 0.05). The expression of COL I A2 mRNA in 160.000 mg/L(0.54 ± 0.01 ) was lower than that of the control group(P ＜ 0.05). Conclusions Fluoride may affect mRNA expression of Osterix and Runx2 in osteoblast and their expression level is related to fluoride concentration.Runx2 and Osterix can also regulate the expression of COL I A2 mRNA. Key words: Fluoride poisoning; Gene expression; Runx2; Osterix; Collagen type Ⅰ

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氟对成骨细胞Runx2、Osterix和COL I A2表达的影响

目的研究氟对体外培养成骨细胞Runx2、Osterix及其下游细胞COL I A2表达的影响。方法体外培养人成骨细胞Saos-2。按氟(NaF)使用剂量0(对照)、0.625、1.250、2.500、5.000、10.000、20.000、40.000、800.000、160.000 mg/L分组。培养24 h后收集细胞，提取RNA，采用荧光定量逆转录聚合酶链反应[Real-time (RT)-PCR]检测Runx2、Osterix及下游基因COL I A2 mRNA表达。结果NaF体外培养24 h后，0.625、1.250、2.500、5.000、10.000、20.000 mg/L组(388.00±41.80、209.00±25.80、42.80±4.52、63.00±16.10、24.30±4.23、16.20±4.32)Runx2表达量均高于对照组(1.00±0.12,P均<0.05)。40000、800.000、160.000 mg/L组Runx2表达量分别为0.40±0.05、1.91±0.28、4.87±1.36，与对照组比较，差异均无统计学意义(P > 0.05)。1.250、2.500、5.000 mg/L组Osterix mRNA表达量(4.04±1.67、229.00±51.00、46.40±10.60)高于对照组(1.00±0.42，均P < 0.05)。Osterix mRNA在10 000、20 000、40 000、80 000、16 000 mg/L组的表达量(0。16±0.07、0.13±0.01、1.73±0.54、0.01±0.01、0.09±0.01)，与对照组比较，差异均无统计学意义(P > 0.05)。0.625、1.250、2.500、5.000、10.000、20.000 mg/L组COL I A2 mRNA表达量(2.27±0.89、8.03±2.31、14.20±2.75、7.66±1.34、8.96±2.30)高于对照组(1.00±0.04，均P < 0.05)。160.000 mg/L浓度组COL I A2 mRNA表达量(0.54±0.01)低于对照组(P < 0.05)。结论氟化物可影响成骨细胞Osterix和Runx2 mRNA的表达，其表达水平与氟化物浓度有关。Runx2和Osterix也能调控COL ia2 mRNA的表达。关键词:氟化物中毒;基因表达;Runx2;Osterix;胶原蛋白类型Ⅰ

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来源期刊

中华地方病学杂志我国对人类健康危害特别严重的地方性疾病：克山病、大骨节病、碘缺乏病、地方性氟中毒、地方性砷中毒、鼠疫、布鲁氏菌病、寄生虫、新冠肺炎等疾病，同时还报道多发性自然疫源性疾病。

CiteScore

1.60

自引率

0.00%

发文量

8714

期刊介绍： The Chinese Journal of Endemiology covers predominantly endemic diseases threatening health of the people in the areas affected by the diseases including Keshan disease, Kaschin-Beck Disease, iodine deficiency disorders, endemic fluorosis, endemic arsenism, plague, epidemic hemorrhagic fever, brucellosis, parasite diseases and the diseases related to local natural and socioeconomic conditions; and reports researches in the basic science, etiology, epidemiology, clinical practice, control as well as multidisciplinary studies on the diseases.