Grain quality indices in common wheat lines with introgressions of chromosome 1U from Aegilops biuncialis Vis.

N. Kozub, I. Sozinov, H. Bidnyk, N. O. Demianova, O. Sozinova, L. Yanse, A. Karelov, Y. Blume
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Abstract

Aim. The aim of this study was to analyze grain quality indices in winter common wheat lines with introgressions of chromosome 1U from Aegilops biuncialis Vis. marked by storage protein loci. Methods. Acid polyacrylamide gel electrophoresis and SDS-electrophoresis of storage proteins were performed to identify introgressions. Grain quality indices (SDS sedimentation volume and grain protein content) were analyzed in lines with introgressed chromosome 1U or its arm 1UL, as well as in the cultivars Panna and Bezostaya 1. Results. SDS-sedimentation volume in the cultivars and lines depended on year’s conditions. The studying of the lines during two years has demonstrated that the presence of the allele at the high molecular weight glutenin subunit locus Glu-U1 from Ae. biuncialis was associated with a high volume of SDS-sedimentation SDS30 (higher than that in the cultivar Bezostaya 1). The introgressive lines show high grain protein content. Conclusions. The effect of the allele at the high molecular weight glutenin subunit locus Glu-U1 from Ae. biuncialis on SDS-sedimentation value is similar to that of the high-quality allele Glu-B1al. The lines with the introgressed allele at Glu-U1 from Ae. biuncialis are valuable initial material for breeding for quality.Keywords: Triticum aestivum, Aegilops biuncialis, high molecular weight glutenin subunits, SDS-sedimentation, protein content.
带1U染色体渗入的普通小麦品系的籽粒品质指标。
的目标。本研究旨在分析以贮藏蛋白位点为标记的双山杨1U染色体渗入的冬小麦品系的籽粒品质指标。方法。用酸性聚丙烯酰胺凝胶电泳和sds电泳鉴定储存蛋白的基因渗入。分析了染色体1U及其旋支1UL的渐渗系以及Panna和Bezostaya 1号的籽粒品质指标(SDS沉降体积和籽粒蛋白质含量)。结果。品种和品系的sds沉降量与年份条件有关。经过2年的研究发现,该等位基因位于Ae的高分子量谷蛋白亚基位点glu1 - u1上。biunalis与高sds沉淀量SDS30相关(高于品种Bezostaya 1)。渐渗系籽粒蛋白质含量高。结论。该等位基因在Ae的高分子量谷蛋白亚基位点Glu-U1上的作用。biuncialis对sds -沉降值的影响与优质等位基因glu - bal相似。来自Ae的Glu-U1等位基因的渐渗系。双孔雀鱼是优良育种的重要原料。关键词:小麦,双歧小麦,高分子量谷蛋白亚基,sds -沉淀法,蛋白质含量
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