VIM, NDM, IMP, GES, SPM, GIM, SIM Metallobetalactamases in Carbapenem-Resistant Pseudomonas Aeruginosa Isolates from a Turkish University Hospital

Y. Tanrıverdi Çaycı, Ilknur Biyik, A. Birinci
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引用次数: 4

Abstract

Background: Pseudomonas aeruginosa is an important opportunistic pathogen, and carbapenem resistance is an emerging problem. The determination of resistance genes is vital for epidemiological purposes, and the early determination of carbapenemase production methods is also recommended. Objectives: The present study aimed to investigate the presence of VIM, NDM, IMP, GES, SPM, GIM, and SIM genes and study the results of the blue carba test as a phenotypic carbapenamase detection method in P. aeruginosa. Methods: In this study, 200 carbapenem-resistant P. aeruginosa isolates were included. The DNA extraction of the carbapenem-resistant isolates was performed using the boiling method. Following the DNA extraction, optimization was conducted using the original primers. After optimization, the VIM, NDM, IMP, GES, SPM, GIM, and SIM genes were examined using the polymerase chain reaction (PCR) method. Results: The isolates were mainly identified from the tracheal aspirate cultures (34.5%). The PCR method revealed the presence of VIM in one of the P. aeruginosa isolates, and the NDM gene in one isolate using. None of the isolates was positive in terms of the IMP, GES, SPM, GIM, and SIM genes. Conclusions: In our study, two carbapenemase genes (VIM and NDM) were detected in the P. aeruginosa isolates.
土耳其大学医院耐碳青霉烯铜绿假单胞菌分离株中的VIM, NDM, IMP, GES, SPM, GIM, SIM金属β -内酰胺酶
背景:铜绿假单胞菌是重要的条件致病菌,碳青霉烯类耐药是一个新兴问题。耐药基因的测定对流行病学研究至关重要,同时也建议早期测定碳青霉烯酶的生产方法。目的:本研究旨在探讨VIM、NDM、IMP、GES、SPM、GIM和SIM基因在铜绿假单胞菌中是否存在,并研究蓝碳水化合物试验作为碳青霉酶表型检测方法的结果。方法:选取200株耐碳青霉烯类铜绿假单胞菌。采用煮沸法提取耐碳青霉烯菌株的DNA。提取DNA后,使用原始引物进行优化。优化后,采用聚合酶链反应(PCR)法检测VIM、NDM、IMP、GES、SPM、GIM和SIM基因。结果:分离株主要来源于气管吸出培养物(34.5%)。PCR方法在铜绿假单胞菌分离株中发现了VIM基因,在铜绿假单胞菌分离株中发现了NDM基因。IMP、GES、SPM、GIM和SIM基因均未检测出阳性。结论:本研究在铜绿假单胞菌分离株中检测到两个碳青霉烯酶基因(VIM和NDM)。
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