The Combination of Decaffeinated Coffee (Coffea canephora) and Green Tea (Camellia sinensis) Reduced PSGL-1 Glycosylation by GALNT2 in Ox-LDL-induced RAW 264.7

Q4 Medicine

International Journal of Nutrition, Pharmacology, Neurological Diseases Pub Date : 2022-07-01 DOI:10.4103/ijnpnd.ijnpnd_7_22

Rohman Mohammad Saifur, Sishartami Lintang Widya, Widodo Nashi, Rachmawati Ermin, Lukitasari Mifetika

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引用次数: 1

Abstract

Context: Coffee and green tea extract combination is expected to reduce macrophage migration. Aims: We investigated the effects of combination of coffee and green tea extracts on GALNT2 gene expression and PSGL-1 protein expression in Ox-LDL-induced RAW 264.7 cells. Materials and methods: RAW 264.7 cells were treated with a combination of coffee and green tea extracts with three different doses for 24 hours (coffee 80 μg/mL + green tea 80 μg/mL; coffee 160 μg/mL + green tea 160 μg/mL; coffee 320 μg/mL + green tea 320 μg/mL), respectively. Then, cells stimulated with 8 μg/mL Ox-LDL for 20 hours. GALNT2 mRNA expression was analyzed by reverse transcription polymerase chain reaction and western blot for PSGL-1 protein expression. Results: GALNT2 gene expression and PSGL-1 protein expression decreased significantly (P < 0.05) with treatment by combination of coffee and green tea extracts in dose-dependent manner. Conclusion: Coffee and green tea extract combination administration significantly reduced GALNT2 gene expression and PSGL-1 protein expression in Ox-LDL-induced RAW 264.7 cells.

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脱咖啡因咖啡(Coffea canephora)和绿茶(Camellia sinensis)联合使用可降低ox - ldl诱导的RAW 264.7中GALNT2介导的PSGL-1糖基化

背景:咖啡和绿茶提取物组合有望减少巨噬细胞的迁移。目的:研究咖啡和绿茶提取物联合使用对ox - ldl诱导的RAW 264.7细胞GALNT2基因表达和PSGL-1蛋白表达的影响。材料与方法:用三种不同剂量的咖啡和绿茶提取物联合作用RAW 264.7细胞24小时(咖啡80 μg/mL +绿茶80 μg/mL;咖啡160 μg/mL +绿茶160 μg/mL;咖啡320 μg/mL +绿茶320 μg/mL)。然后用8 μg/mL Ox-LDL刺激细胞20小时。采用逆转录聚合酶链反应和western blot检测PSGL-1蛋白的表达。结果:GALNT2基因表达和PSGL-1蛋白表达在咖啡和绿茶提取物联合作用下显著降低(P < 0.05)，且呈剂量依赖性。结论:咖啡和绿茶提取物联合给药可显著降低ox - ldl诱导的RAW 264.7细胞GALNT2基因表达和PSGL-1蛋白表达。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Nutrition, Pharmacology, Neurological Diseases Medicine-Pharmacology (medical)

CiteScore

0.80

自引率

0.00%

发文量

期刊介绍： The International Journal of Nutrition, Pharmacology, Neurological Diseases (IJNPND) is an international, open access, peer reviewed journal which covers all fields related to nutrition, pharmacology, neurological diseases. IJNPND was started by Dr. Mohamed Essa based on his personal interest in Science in 2009. This journal doesn’t link with any society or any association. The co-editor-in chiefs of IJNPND (Prof. Gilles J. Guillemin, Dr. Abdur Rahman and Prof. Ross grant) and editorial board members are well known figures in the fields of Nutrition, pharmacology, and neuroscience. First, the journal was started as two issues per year, then it was changed into 3 issues per year and since 2013, it publishes 4 issues per year till now. This shows the slow and steady growth of this journal. To support the reviewers and editorial board members, IJNPND offers awards to the people who does more reviews within one year. The International Journal of Nutrition, Pharmacology, Neurological Diseases (IJNPND) is published Quarterly. IJNPND has three main sections, such as nutrition, pharmacology, and neurological diseases. IJNPND publishes Research Papers, Review Articles, Commentaries, case reports, brief communications and Correspondence in all three sections. Reviews and Commentaries are normally commissioned by the journal, but consideration will be given to unsolicited contributions. International Journal of Nutrition, Pharmacology, Neurological Diseases is included in the UGC-India Approved list of journals.