Determination of Cefuroxime Axetil in Tablet by HPLC, UV and First-Order Derivative Spectroscopy Methods & Plasma by UV Spectroscopy Method

A. Turan, Y. Kadioglu
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Abstract

It was aimed to develop and validate the UV spectroscopic, the first-order derivative spectroscopy, and the HPLC method for determination of cefuroxime axetil in bulk and tablets and also in spiked human plasma samples by UV spectroscopy method. In the spectroscopic analyses, the maximum absorbance of cefuroxime axetil in acetonitrile was obtained at 277 nm wavelength in the spectra. In first-order derivative spectroscopy method, two peaks were observed in spectra, a maximum at 258 nm and a minimum at 298 nm. 298 nm wavelength was used in the study. In HPLC-UV study, parameters were chosen as follows: C18 column, 0.1% acetic acid-acetonitrile (30:70; v/v) for mobile phase, 1.0 mL/min of flow rate, 280 nm of wavelength, 10 μL of injection volume and etodolac (2.5 μg/mL) as internal standard. Accuracy, precision, recovery, linearity and sensitivity parameters were determined for each of the three methods. Developed and validated methods were successfully applied on 4 tablets which are named as Cefaks, Cefurol, Aksef ve Enfexia. As a result, it is claimed that proposed method is sensitive, precise, accurate, and successfully used in quality control studies in the drug industry.
高效液相色谱法、紫外光谱法和一阶导数光谱法测定片中头孢呋辛酯的含量,血浆中头孢呋辛酯的含量
建立并验证了紫外分光光度法、一阶导数分光光度法和高效液相色谱法测定头孢呋辛酯原料药、片剂和加标血浆样品的含量。在光谱分析中,头孢呋辛酯在乙腈中的最大吸光度在277 nm波长处。一阶导数光谱法在258 nm处有两个峰,在298 nm处有一个峰。实验波长为298nm。在HPLC-UV研究中,选择的参数如下:C18柱,0.1%醋酸-乙腈(30:70;v/v)为流动相,流速1.0 mL/min,波长280 nm,进样量10 μL,内标为依托度酸(2.5 μg/mL)。测定了三种方法的准确度、精密度、回收率、线性度和灵敏度等参数。建立并验证的方法成功应用于头孢克、头孢罗尔、阿克塞夫和恩非昔亚4种片剂。结果表明,该方法灵敏、精确、准确,可成功应用于医药行业的质量控制研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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