Immunofluorescence Patterns in Selected Dermatoses, Including Blistering Skin Diseases Utilizing Multiple Fluorochromes

Ana Maria Abreu Velez, Juliana Calle-Isaza, M. S. Howard
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引用次数: 3

Abstract

Background: Autoimmune vesiculobullous disorders represent a heterogeneous group of dermatoses whose diagnosis is made based on clinical history, histologic features, and immunopathologic features. The most commonly used techniques for the diagnosis of these diseases are direct and indirect immunofluorescence (DIF and IIF), including salt-split processing. NaCl split skin is used to determine the level of blister formation, and the localization of autoantibodies relative to the split. Classically, immunofluorescence has been performed with one fluorochrome in the diagnosis of autoimmune bullous skin diseases. Aims: To compare DIF and IIF of the skin, using a single fluorochrome versus multiple fluorochromes. Materials and Methods: We studied 20 autoimmune skin disease cases using fluorescein isothiocyanate (FITC) alone, in comparison to multiple fluorochromes (with or without DNA counterstaining). Results: The use of multiple fluorochromes helped to simultaneously visualize reactivity in multiple skin areas, in contrast to using FITC alone. Conclusions: Using multiple fluorochromes allows simultaneous labeling of two or more antigens within the same cell/or tissue section, assists in colocalization of unknown antigens with known molecules, and helps in ruling out "background" staining.
免疫荧光模式在选定的皮肤病,包括疱性皮肤病利用多种荧光色
背景:自身免疫性囊泡性疾病是一种异质性皮肤病,其诊断是基于临床病史、组织学特征和免疫病理特征。诊断这些疾病最常用的技术是直接和间接免疫荧光(DIF和IIF),包括盐裂处理。NaCl裂皮用于测定水疱形成的程度,以及相对于裂口的自身抗体的定位。经典的,免疫荧光已与一个荧光染料在自身免疫性大疱性皮肤病的诊断。目的:比较使用单一荧光染料和多种荧光染料的皮肤DIF和IIF。材料和方法:我们单独使用异硫氰酸荧光素(FITC)研究了20例自身免疫性皮肤病病例,并与多种荧光色素(有或没有DNA反染色)进行了比较。结果:与单独使用FITC相比,使用多种荧光染料有助于同时观察多个皮肤区域的反应性。结论:使用多种荧光染料可以同时标记同一细胞/组织切片内的两种或两种以上抗原,有助于将未知抗原与已知分子共定位,并有助于排除“背景”染色。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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