A Microneedle-Microplate Platform to Detect Biomarkers in the Skin

Abstract

This research aimed to integrate an established signalquantification technique with immunocapture microneedle arrays (MNAs) to improvebiomarker-based disease diagnostics in the skin. In this miniaturized sandwichenzyme-linked immunosorbent assay (ELISA) platform, the capture antibody wasimmobilised onto the surface of microneedles which, when inserted superficiallyinto porcine skin, captured endogenous porcine immunoglobulin G (IgG) intradermally.Signal development was achieved by incubating the microneedles ino-phenylenediamine (OPD) solution in a 384-well microplate and measuring theabsorbance in a microplate spectrophotometer at 450 nm. This technique allowsfor rapid biomarker detection with high-throughput processing. Immunocapture microneedledevices such as these can be easily adapted for targeting different biomarkersor multiplexed leaving plenty of scope for future work and assay optimisation.