The Electrochemical Impedance Recognition for Two Heme Proteins on dsDNA/PGA/GCE

Abstract

A DNA-modified electrode was fabricated by the way of electrode modifying, namely the polymerisation-covalently bonding, for studying the recognition of DNA to heme protein. Electrochemical Impedence Resistance (EIS) showed that electron transfer resistance increased with the adding of Hemoglobin (Hb) and Cytochrome c (Cyt c). Reaction constant of Hb and DNA is K=1.13×10⁵ L-mol^-1, and K=4.08×10⁵ L-mol^-1 for Cyt c, indicating that DNA-modified electrode can well recognize Hb and Cyt c.