Study on Culture of Human Dental Pulp Stem Cells to Apply in Tissue Engineering

Journal of Biomimetics, Biomaterials and Tissue Engineering Pub Date : 2011-09-01 DOI:10.4028/www.scientific.net/JBBTE.11.13

Tran Le Bao Ha, Doan Nguyen Vu, T. Quan, Ngoc Phan Kim, Hung Hoang Tu, Bao Tram Hoang Dao, Ngoc Mai Dang Vu, T. Nguyen

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引用次数: 7

Abstract

Dental pulp cell research might open a promising application in tooth tissue regeneration. The aim of this study is to establish a protocol for in vitro culture the human dental pulp stem cells to apply in tissue engineering. Human premolar and impacted third molars were collected and disinfected. Dental pulp fragments were cultured with Dulbecco's Modified Eagle Medium: Nutrient Mixture F-12 (DMEM/F12) medium supplemented with 10% Fetal Bovine Serum (FBS). Dental pulp stem cells (DPSCs) were identified using proliferation assay, RT-PCR and flow cytometry. Growth of DPSCs on dentin surface was assessed by MTT assay. The study showed that we successfully isolated, cultured and characterized dental pulp cells by outgrowth method. Cultured population of cells expressed in high level of Oct4, CD146, CD90, CD44. DPSCs proliferated on chemically and mechanically treated dentin surface. This research provides important information and a basis for further investigations to establish dental tissue engineering protocols.

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人牙髓干细胞培养在组织工程中的应用研究

牙髓细胞的研究在牙组织再生方面具有广阔的应用前景。本研究旨在建立人牙髓干细胞的体外培养方案，并将其应用于组织工程。收集人前磨牙和阻生第三磨牙并消毒。用Dulbecco's Modified Eagle培养基:营养混合物F-12 (DMEM/F12)培养基添加10%胎牛血清(FBS)培养牙髓碎片。采用增殖试验、RT-PCR和流式细胞术对牙髓干细胞进行鉴定。MTT法观察牙本质表面DPSCs的生长情况。研究表明，我们成功地分离、培养了牙髓细胞，并对其进行了表征。培养高水平表达Oct4、CD146、CD90、CD44的细胞群。DPSCs在化学处理和机械处理的牙本质表面增殖。本研究为进一步研究建立口腔组织工程规程提供了重要信息和基础。

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Journal of Biomimetics, Biomaterials and Tissue Engineering

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