Comparative Evaluation of Biological Activities, Bioautography and Spot Screening of Boiled and Macerated Extracts of Medicinal Plants against Zoonotic Pathogens

British microbiology research journal Pub Date : 2016-01-10 DOI:10.9734/BMRJ/2016/24654

R. Ilyas, S. Andleeb, M. Babar, R. Mustafa, Irsa Shafique, A. Zafar, Shaukat Ali

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Abstract

Aims: There is an escalating requirement for the development of novel drugs for the treatment of zoonotic diseases. Herbal plants have always been the best source of therapeutic agents. Study Design: Biological activities of medicinal plants. and 29 June, 2015. Methodology: In current research work biological properties of various extracts of medicinal plants i.e. Atropa acuminate, Atropa belladonna and Morus nigra were investigated. Extracts were prepared through both maceration and boiling extraction methods. Antibacterial activity against zoonotic pathogens such as Serratia odorifera , Enterobacter amnigenus , Shigella flexneri, Salmonella Typhimurium and Pseudomonas aeruginosa was assessed using agar well diffusion method. DPPH free radical scavenging method was used to measure antioxidant potential. Total phenolic and flavonoid contents were estimated. Results: It was found that boiling extracts showed maximum antibacterial activity against all tested pathogens. The results of antioxidant potential revealed the maximum activity in root extracts of A. belladonna and all extracts of M. nigra while A. acuminata showed lowest scavenging activity. Phytochemical analysis indicated the existence of alkaloids, flavonoids, quinones, phenols, tannins, terpenoides, glycosides, steroids and carbohydrates. Thin layer chromatography (TLC) also mentioned the presence of phytochemical constituents. TLC- developed plates have showed the maximum presence of both antioxidant constituents and flavonoids contents in all boiling extracts of Z. armatum . Bio-autobiography by agar overlying assay indicated the significant inhibition of tested pathogens. Conclusion: The current study provides scientific confirmation supporting the therapeutic potency of these medicinal plants for medicinal uses and identifies gaps for future research to facilitate commercial utilization.

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药用植物水煮提取物和浸渍提取物抗人畜共患病原菌生物活性比较评价、生物自传和现场筛选

目的:人畜共患疾病的治疗对新药的开发要求越来越高。草本植物一直是治疗药物的最佳来源。研究设计:药用植物的生物活性。2015年6月29日。方法:在目前的研究工作中，对不同药用植物——尖叶Atropa、颠茄Atropa和桑葚(Morus nigra)提取物的生物学特性进行了研究。采用浸渍法和沸煮法制备提取物。采用琼脂孔扩散法测定对臭气沙雷菌、羊水肠杆菌、福氏志贺氏菌、鼠伤寒沙门菌和铜绿假单胞菌等人畜共患病原菌的抑菌活性。采用DPPH自由基清除法测定抗氧化电位。测定总酚和类黄酮含量。结果:水煮提取物对所有病原菌的抑菌活性均最高。抗氧化活性测定结果显示，颠茄根提取物和黑草提取物的抗氧化活性最高，而荆芥提取物的抗氧化活性最低。植物化学分析表明，其中含有生物碱、黄酮类、醌类、酚类、单宁类、萜类、苷类、类固醇和碳水化合物。薄层色谱(TLC)也提到了植物化学成分的存在。薄层色谱分析结果表明，各水煮提取物中抗氧化成分和黄酮类化合物含量均最高。琼脂复盖生物自传实验表明，对被试病原菌有明显的抑制作用。结论:本研究为这些药用植物的药用价值提供了科学依据，并为今后的研究发现了空白，以促进商业利用。

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British microbiology research journal

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