Extraction of Flavonoids from Sword Flower and Methodology of Its Preparations

Abstract

Qixing Jianhua (Hylocerei Undati Flos) is not only a specialty plant of Zhaoqing, but also has a lot of attention because it contains a variety of active ingredients. In order to explore the role of flavonoids in the seven-star sword-flower, by designing single factor test and L9 (Fourth power of 3) orthogonal test, the effects of different extraction time, ethanol concentration and particle size on the total flavonoids yield and process optimization were studied. After ultrasonic and 95% alcohol extraction, followed by ethyl acetate extraction, thin plate chromatography and other identification. Antioxidant ability analysis of the extract was carried out by salicylic acid method and antimicrobial test. And then the moisturizing anti-oxidation cream was prepared by using sword-flower alcohol extract. The results showed that the extraction rate was highest at 95% ethanol concentration, 1:60 of material to liquid ratio, 60 min extraction time, and 70 degrees Celsius extraction temperature, reaching 13.41 mg/g. The antibacterial effect of the extract is obvious, and it has obvious scavenging effect of hydroxyl radicals. Therefore, this paper has important theoretical and practical significance for the further study and application of flavonoids in the future. It can be predicted that the flavonoids extracted from Qixing sword flower, a kind of traditional Chinese medicine in Zhaoqing, will be widely used in the fields of food, medicine and health in the future.