Serological and genetic analysis of ABO ambiguous blood group samples

Blood and genomics Pub Date : 2020-01-01 DOI:10.46701/bg.2020022020112

Ying Li, Jinming Xu, Yongliang Zhu, Ting Xiong, Ling Zhao, Yunnan Yao, Xiaoyu Zhou

{"title":"Serological and genetic analysis of ABO ambiguous blood group samples","authors":"Ying Li, Jinming Xu, Yongliang Zhu, Ting Xiong, Ling Zhao, Yunnan Yao, Xiaoyu Zhou","doi":"10.46701/bg.2020022020112","DOIUrl":null,"url":null,"abstract":"The accuracy of regular serum methods to detect ABO blood groups can be negatively affected by some factors, such as irregular antibodies, autoantibodies or effects of diseases leading to false or weak agglutination. This study aimed to accurately identify ambiguous ABO blood groups by serological and gene detection methods. The samples were collected in the First Affiliated Hospital of Nanjing Medical University from December 2018 to December 2019. ABO genotyping was performed by polymerase chain reaction-sequence specific primer (PCR-SSP) method in 20 samples, and ABO exons 6 and 7 or FUT1 and FUT2 genes were sequenced in 5 samples. The genes detected in the 21 specimens included 4 cases of A/B, 2 cases of A205/O01, 3 cases of A/O01, 3 cases of A/O02, 1 case of O01/O01, 1 case of O01/O02, 1 case of B/O01, 1 case of B/O02, 1 case of Bel/O01, 1 case of Cisab01/O01, 1 case of rare B/O04, 1 case of Bombay-like Bmh, 1 case of new gene showing c.261del G of exon 6, c.579 T>C of exon 7 and B new/O01. This study suggests that ABO blood group genotyping technology combined with serological typing can be used for accurately typing ambiguous blood groups.","PeriodicalId":87226,"journal":{"name":"Blood and genomics","volume":"1 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Blood and genomics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.46701/bg.2020022020112","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 1

Abstract

The accuracy of regular serum methods to detect ABO blood groups can be negatively affected by some factors, such as irregular antibodies, autoantibodies or effects of diseases leading to false or weak agglutination. This study aimed to accurately identify ambiguous ABO blood groups by serological and gene detection methods. The samples were collected in the First Affiliated Hospital of Nanjing Medical University from December 2018 to December 2019. ABO genotyping was performed by polymerase chain reaction-sequence specific primer (PCR-SSP) method in 20 samples, and ABO exons 6 and 7 or FUT1 and FUT2 genes were sequenced in 5 samples. The genes detected in the 21 specimens included 4 cases of A/B, 2 cases of A205/O01, 3 cases of A/O01, 3 cases of A/O02, 1 case of O01/O01, 1 case of O01/O02, 1 case of B/O01, 1 case of B/O02, 1 case of Bel/O01, 1 case of Cisab01/O01, 1 case of rare B/O04, 1 case of Bombay-like Bmh, 1 case of new gene showing c.261del G of exon 6, c.579 T>C of exon 7 and B new/O01. This study suggests that ABO blood group genotyping technology combined with serological typing can be used for accurately typing ambiguous blood groups.

查看原文本刊更多论文

ABO歧义血型样本的血清学和遗传学分析

常规血清法检测ABO血型的准确性会受到一些因素的负面影响，如不规则抗体、自身抗体或导致假或弱凝集的疾病的影响。本研究旨在通过血清学和基因检测方法准确鉴定模棱两可的ABO血型。样本于2018年12月至2019年12月在南京医科大学第一附属医院采集。采用聚合酶链反应-序列特异性引物(PCR-SSP)法对20份样本进行ABO基因分型，对5份样本的ABO 6、7外显子或FUT1、FUT2基因进行测序。21例标本中检出的基因包括:A/B型4例、A205/O01型2例、A/O01型3例、A/O02型3例、O01/O01型1例、O01/O02型1例、B/O01型1例、B/O02型1例、Bel/O01型1例、Cisab01/O01型1例、罕见B/O04型1例、bombay样Bmh型1例、6外显子c.261del G型1例、c.579型1例外显子7的T>C和B new/O01。本研究提示，ABO血型基因分型技术与血清学分型相结合，可以对歧义血型进行准确分型。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Blood and genomics

自引率

0.00%

发文量