In vitro biological control of Colletotrichum gloeosporioides ,causal organism of anthracnose of sarpagandha (Roulvolfia serpentina )

Abstract

Five isolates of Trichoderma viride and two isolates of Beauveria bassiana were screened against Colletotrichum gloeosporioides for their antagonistic potentiality by dual culture plate method. Three isolates of T. viride and one isolate of Beauveria bassiana were class –1 antagonists. Among the class -1 antagonists ,T1 isolate of T. viride showed best hyperparasitic activity( 3.30 cm ) followed by T10 ( 3.10cm ) ,T12 ( 3.00 cm) and BB1 of B.bassiana( !.20 cm). The selected cultures from pairing of mycoparasitized pathogen were observed under microscope ( Leitz Laborlux K , Germany ) to study of hyphal interactions between the antagonist and the pathogen and photographed when required. Therefore, this in vitro study strongly suggested that T. viride can be applied in field trial (in vivo ) as effective biocontrol agents against anthracnose of sarpagandha ( C. O. Colletotrichum gloeosporioides).