Gastric mast cell population in rats under ground-simulated weightlessness conditions and in early readaptation period

A. A. Zhukov, N. Alexeeva, V. V. Shishkina, T. V. Samoilenko, E. S. Goryushkina, S. Zolotareva, S. A. Budnevskaya, E. E. Ivanova, S. Klochkova
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Abstract

   The effect of altered gravity on the digestive system is one of the challenging issues in space biology and medicine. The study of the effect of weightlessness on the mast cell (MC) functioning in the gastrointestinal organs is determined by their multifunctionality and participation in the developing adaptive and pathological processes.   The aim of the study was to investigate the morphofunctional state of MCs in the stomach membranes of Wistar rats during 14 days of ground-based simulated weightlessness conditions and in a 3-day recovery period.   Material and methods. The experiment involved 3 groups of Wistar rats: control, experimental – exposed to antiorthostatic suspension – and a 3-day-recovery group, 7 animals each, respectively. Identification of MCs was carried out using May-Grunwald staining with additional staining with Giemsa solution and immunohistochemical tryptase detection. The results obtained were statistically processed with SPSS 13 using parametric and non-parametric criteria.   Results. A significantly increased number of metachromatic MC population was found only in the mucosa in animals of the antiorthostatic suspension and 3-day-recovery groups compared to the vivarium control. There was a sharp decrease in degranulating forms of MCs in the submucosalcoat in animals of the 3-day-recovery group compared to the control. A significantly increased number of degranulating forms of MCs was revealed in the muscle membrane in animals of the antiorthostatic suspension group. Immunohistochemical staining of MCs with antibodies to tryptase, in contrast to staining for metachromasia, allowed identifying a significant representation of tryptase-positive cells in the gastric mucosa in animals of the 3-day-recovery group.   Conclusion. The results obtained support gravisensitivity of certain structural components of the stomach, and also evidence MC and their secretome participation in the developing adaptive responses to the effects of altered gravity both at the cellular and tissue levels.
地面模拟失重和早期适应条件下大鼠胃肥大细胞群的变化
重力改变对消化系统的影响是空间生物学和医学中具有挑战性的问题之一。失重对胃肠道肥大细胞功能影响的研究是由肥大细胞的多功能性和参与发育中的适应性和病理过程决定的。本研究的目的是研究Wistar大鼠在14天的地面模拟失重条件和3天的恢复期胃膜MCs的形态功能状态。材料和方法。实验采用Wistar大鼠3组:对照组、实验组(抗立悬浮液)和恢复期3天组,每组7只。MCs鉴定采用May-Grunwald染色,外加Giemsa溶液染色和免疫组化胰蛋白酶检测。所得结果用SPSS 13采用参数和非参数标准进行统计处理。结果。与体内对照组相比,仅在抗立悬浮液组和3天恢复组动物的粘膜中发现了显着增加的异色MC种群数量。与对照组相比,3天恢复组动物的粘膜下涂层中MCs的脱颗粒形式急剧减少。抗立悬浮液组动物肌膜中脱颗粒形式的MCs数量显著增加。用胰蛋白酶抗体对MCs进行免疫组织化学染色,与异色染色相比,可以在3天恢复组动物的胃粘膜中识别出胰蛋白酶阳性细胞的显著代表性。结论。研究结果支持了胃某些结构成分的重力敏感性,也证明了MC及其分泌组在细胞和组织水平上参与了对重力改变影响的适应性反应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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