KARAKTERISASI METABOLIT SEKUNDER DARI DAUN MANGROVE Rhizopora stylosa SEBAGAI ANTIOKSIDAN (CHARACTERIZATION OF SECONDARY METABOLITE MANGROVE LEAVES Rhizopora stylosa SPECIES AS ANTIOXIDANTS)

Septian Triadi Syahputra, A. Sapar, Ari Widiyantoro
{"title":"KARAKTERISASI METABOLIT SEKUNDER DARI DAUN MANGROVE Rhizopora stylosa SEBAGAI ANTIOKSIDAN (CHARACTERIZATION OF SECONDARY METABOLITE MANGROVE LEAVES Rhizopora stylosa SPECIES AS ANTIOXIDANTS)","authors":"Septian Triadi Syahputra, A. Sapar, Ari Widiyantoro","doi":"10.26418/indonesian.v5i1.53566","DOIUrl":null,"url":null,"abstract":"Mangrove leaves or also known as “bakau” by the people of Mempawah Regency, which has (Rhizopora stylose) species containing alkaloids, flavonoids, steroids, terpenoids, phenolics, and saponins that can be used as antioxidant compounds. This study was aimed to determine the antioxidant activity of extracts and secondary metabolite fractions, and also to determine the characteristics of isolate compounds from mangrove leaves (Rhizopora stylose). Maceration of 1.57 kg of dry powder of mangrove leaves (Rhizopora stylose) with methanol as a solvent obtained a crude extract of 141.13 g of methanol with a percent yield of 8.98%. Partitioning the crude methanol extract obtained the n-hexane, dichloromethane, ethylacetate, and residue fractions by weight and percent yield, respectively, namely 6.95 g (0.44%), 26.74 g (1.70%), 26 .66 (1.69%), and 25.75 g (1.64%). The results of the antioxidant activity test showed that the dichloromethane fraction had a better antioxidant activity with an IC50 value of 121.314 g/mL. Separation and purification of the dichloromethane fraction to obtain isolates were carried out by column chromatography in order to obtain pure isolates which were characterized by the appearance of a single spot on the tested plate with various eluents, namely, isolate F7. The 1H-NMR data for isolate F7 showed chemical shifts for aromatic protons of 8.52 ppm, 7.70 ppm, 7.67 ppm, protons for tertiary C of 5.41 ppm, and protons for methyl and methylene of 3.50 ppm, 3.47 ppm, 1.59 ppm, 1.23 ppm, 1.05 ppm, and 0.84 ppm. H-NMR data showed that isolate F7 had similarities with the chemical shift of p-Oxy-2-Ethylhexyl benzaldehyde, namely 7.71 ppm, 7.54 ppm, 4.22 ppm, 1.68 ppm, 1.35 ppm, 1.32 ppm, 1.30 ppm, 0.90 ppm but different in the alkoxy substituent position from isolate F7 which was in the meta position","PeriodicalId":13554,"journal":{"name":"Indonesian Journal of Pure and Applied Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2022-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Indonesian Journal of Pure and Applied Chemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.26418/indonesian.v5i1.53566","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Mangrove leaves or also known as “bakau” by the people of Mempawah Regency, which has (Rhizopora stylose) species containing alkaloids, flavonoids, steroids, terpenoids, phenolics, and saponins that can be used as antioxidant compounds. This study was aimed to determine the antioxidant activity of extracts and secondary metabolite fractions, and also to determine the characteristics of isolate compounds from mangrove leaves (Rhizopora stylose). Maceration of 1.57 kg of dry powder of mangrove leaves (Rhizopora stylose) with methanol as a solvent obtained a crude extract of 141.13 g of methanol with a percent yield of 8.98%. Partitioning the crude methanol extract obtained the n-hexane, dichloromethane, ethylacetate, and residue fractions by weight and percent yield, respectively, namely 6.95 g (0.44%), 26.74 g (1.70%), 26 .66 (1.69%), and 25.75 g (1.64%). The results of the antioxidant activity test showed that the dichloromethane fraction had a better antioxidant activity with an IC50 value of 121.314 g/mL. Separation and purification of the dichloromethane fraction to obtain isolates were carried out by column chromatography in order to obtain pure isolates which were characterized by the appearance of a single spot on the tested plate with various eluents, namely, isolate F7. The 1H-NMR data for isolate F7 showed chemical shifts for aromatic protons of 8.52 ppm, 7.70 ppm, 7.67 ppm, protons for tertiary C of 5.41 ppm, and protons for methyl and methylene of 3.50 ppm, 3.47 ppm, 1.59 ppm, 1.23 ppm, 1.05 ppm, and 0.84 ppm. H-NMR data showed that isolate F7 had similarities with the chemical shift of p-Oxy-2-Ethylhexyl benzaldehyde, namely 7.71 ppm, 7.54 ppm, 4.22 ppm, 1.68 ppm, 1.35 ppm, 1.32 ppm, 1.30 ppm, 0.90 ppm but different in the alkoxy substituent position from isolate F7 which was in the meta position
红树林的叶子,也被曼帕瓦县的人们称为“巴卡乌”,其中含有(根孢杆菌)物种,其中含有生物碱、类黄酮、类固醇、萜类、酚类物质和皂苷,这些物质可以用作抗氧化化合物。本研究旨在测定红树叶(Rhizopora stylose)提取物和次生代谢产物的抗氧化活性,以及分离化合物的特性。将1.57 kg的红树叶干粉(Rhizopora stylose)以甲醇为溶剂浸渍,得到粗提物为141.13 g甲醇,收率为8.98%。对粗甲醇提取物进行分馏,得到正己烷组分、二氯甲烷组分、乙酸乙酯组分和残渣组分,分别为6.95 g(0.44%)、26.74 g(1.70%)、26.66 g(1.69%)和25.75 g(1.64%)。抗氧化活性试验结果表明,二氯甲烷组分具有较好的抗氧化活性,IC50值为121.314 g/mL。采用柱层析法对二氯甲烷馏分分离纯化得到分离物,得到的分离物在不同洗脱液的检测板上呈现单一斑点,即分离物F7。分离物F7的1H-NMR数据显示芳香质子为8.52 ppm、7.70 ppm、7.67 ppm,叔碳质子为5.41 ppm,甲基和亚甲基质子为3.50 ppm、3.47 ppm、1.59 ppm、1.23 ppm、1.05 ppm和0.84 ppm。H-NMR数据表明,分离物F7与对氧-2-乙基己基苯甲醛的化学位移相似,分别为7.71 ppm、7.54 ppm、4.22 ppm、1.68 ppm、1.35 ppm、1.32 ppm、1.30 ppm、0.90 ppm,但在烷氧取代基位置上与分离物F7不同
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信