Rice Husk Ash as an Adsorbent for Isolated the Celephthalides Compounds from Root of Apium Graveolens Linn for Inhibited Phytopathogenic Fungi in Plants

Abstract

Apium graveolens Linn. is in Apiaceae family, the common name is A. graveolens or celeric. Chemical constituents of A. graveolens found sedanolide, celerin, celephthalide and flavonoids compounds which are repellent mosquito, antioxidant and antibacterial. This study used the root of A. graveolens were extracted by methanol. The crude methanol extracted isolated by the stationary phase of rice husk ash (RHA). Modified the powders of RHA by sieve sizes of Mesh 20, 40 and 100 gave three particle size are 660, 382 and 143 μm respectively, and cleaned by sodium hydroxide. The crude methanol was isolated by each of the particle size of RHA gave F1-F3 and were examined chemical constituents by thin layer chromatography. The fungicides used Rhizopus nigricans is a fungus commonly known as black bread. The orchid farms in Thailand found R. nigricans damaged leaves and roots of orchids. F1-F3 were tested by antifungal assay, as disc diffusion dilution, turbidity cell density is measurement by % transmitant at 625 nm by ultra-violet spectrometer and minimum fungicidal concentration (MFC). F1-F3 were inhibited fungicides in 68, 53 and 21 μg/ml, respectively. The aimed of this study used RHA from the rice-bran oil industry were absorbent for isolated the phytochemical compounds from A. graveolens which have the anti-fungicidal activity.