Replicase-derived resistance against pea early browning virus in Nicotiana benthamiana is an unstable resistance based upon posttranscriptional gene silencing.

IF 1.6 3区物理与天体物理 Q3 PHYSICS, FLUIDS & PLASMAS

Plasma Science & Technology Pub Date : 2001-02-01 DOI:10.1094/MPMI.2001.14.2.196

T van den Boogaart, F Wen, J W Davies, G P Lomonossoff

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引用次数: 19

Abstract

Virus resistance in Nicotiana benthamiana plants containing a translatable Pea early browning virus (PEBV) 54K sequence from the 201K replicase gene has been reported previously. Resistant plants contain multiple transgene copies divided between two loci. Analysis of a genetic series containing the two loci in separate homozygous or heterozygous condition suggest that only one of the loci is necessary to induce the resistance. The resistance observed in R2 and R3 generations of lines containing both transgene loci in homozygous condition became less consistent in R4 and R5 generations. This inversely correlated with steady-state transgene transcript levels of the segregating populations. The use of recombinant Potato virus X vectors carrying PEBV 54K sequences showed that the resistance is based upon posttranscriptional gene silencing, is non-strand specific, and recognizes 3' located sequences within the PEBV 54K sequence.

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复制酶衍生的抗豌豆早褐化病毒性是一种基于转录后基因沉默的不稳定抗性。

此前曾有报道称，含有可翻译的豌豆早期褐变病毒（PEBV）54K 序列（来自 201K 复制酶基因）的烟草植物具有病毒抗性。抗性植株含有多个转基因拷贝，分属两个基因位点。对含有这两个基因位点的基因系列进行分析后发现，只有其中一个基因位点能诱导抗性。在 R2 和 R3 代中观察到的两个转基因位点同源的抗性在 R4 和 R5 代中变得不那么一致。这与分离群体的稳态转基因转录本水平成反比。使用携带 PEBV 54K 序列的重组马铃薯病毒 X 载体表明，抗性是基于转录后的基因沉默，是非链特异性的，并能识别 PEBV 54K 序列中位于 3' 的序列。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plasma Science & Technology 物理-物理：流体与等离子体

CiteScore

3.10

自引率

11.80%

发文量

3773

审稿时长

3.8 months

期刊介绍： PST assists in advancing plasma science and technology by reporting important, novel, helpful and thought-provoking progress in this strongly multidisciplinary and interdisciplinary field, in a timely manner. A Publication of the Institute of Plasma Physics, Chinese Academy of Sciences and the Chinese Society of Theoretical and Applied Mechanics.