Evaluation of Prophylactic and Therapeutic Roles of NAcetylcysteine on Biochemical and Oxidative Changes Induced by Acute Poisoning of Diazinon in Various Rat Tissues

Current Chemical Biology Pub Date : 2020-11-19 DOI:10.2174/2212796814999200818094328

K. Tahmasebi, M. Jafari, F. Izadi, A. Asgari, H. Bahadoran, Javad Heydari, Saeed Khazaie

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引用次数: 3

Abstract

Exposure to diazinon (DZN) as an organophosphorus insecticide is associated with reducing the antioxidant capacity of cells. N-acetyl cysteine (NAC) is widely used in clinics to treat several diseases related to oxidative stress. The current study was aimed to evaluate the prophylactic and therapeutic roles of NAC on biochemical and oxidative changes induced by acute poisoning of DZN in various tissues of male Wistar rats. Thirty rats were divided into five groups: control group received corn oil as DZN solvent; DZN group received 100 mg/kg of DZN; NAC group received 160 mg/kg of NAC; NAC-DZN and DZN-NAC groups received 160 mg/kg of NAC before and after 100 mg/kg of DZN injection, respectively. Plasma and various tissues were prepared and evaluated for the measurement of the biochemical parameters and oxidative stress biomarkers. Both prophylactic and therapeutic treatments by NAC ameliorated the increased lipid peroxidation and decreased glutathione level and superoxide dismutase, catalase and glutathione S-transferase activities in tissues (P<0.05). Moreover, treatment with the NAC caused a significant reduction in DZN-induced high levels of plasma biochemical parameters. Furthermore, acetylcholinesterase activity was positively correlated with both LDH (P=0.000) activity and GSH (P=0.001) level and negatively correlated with MDA (P=0.009) level in the brain. Results suggest that NAC could effectively ameliorate the DZN-induced oxidative stress and cholinergic hyperactivity in various tissues especially in the brain, through free radicals scavenging and GSH synthesis. Prophylactic approach exerted a stronger protective effect compared to a therapeutic treatment.

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nacetyl半胱氨酸对二嗪嗪急性中毒大鼠各组织生化及氧化变化的防治作用

暴露于二嗪农(DZN)作为一种有机磷杀虫剂与降低细胞的抗氧化能力有关。n -乙酰半胱氨酸(NAC)广泛应用于临床治疗多种与氧化应激相关的疾病。本研究旨在探讨nac对雄性Wistar大鼠急性DZN中毒引起的各组织生化和氧化变化的预防和治疗作用。30只大鼠分为5组:对照组以玉米油为溶剂;DZN组给予100 mg/kg DZN;NAC组注射160 mg/kg NAC, NAC-DZN组和DZN-NAC组分别在注射100 mg/kg DZN前后注射160 mg/kg NAC。制备血浆和各种组织并评估其生化参数和氧化应激生物标志物的测量。NAC预防和治疗均能改善组织中脂质过氧化升高、谷胱甘肽水平降低、超氧化物歧化酶、过氧化氢酶和谷胱甘肽s -转移酶活性降低(P<0.05)。此外，用nna处理导致dzn诱导的高水平血浆生化参数显著降低。脑内乙酰胆碱酯酶活性与LDH(P=0.000)活性和GSH (P=0.001)水平呈正相关，与MDA (P=0.009)水平呈负相关。结果表明，NAC可以通过清除自由基和合成GSH，有效改善dzn诱导的各组织氧化应激和胆碱能亢进，尤其是大脑。与治疗性治疗相比，预防性方法具有更强的保护作用。

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来源期刊

Current Chemical Biology Medicine-Biochemistry (medical)

CiteScore

1.40

自引率

0.00%

发文量

期刊介绍： Current Chemical Biology aims to publish full-length and mini reviews on exciting new developments at the chemistry-biology interface, covering topics relating to Chemical Synthesis, Science at Chemistry-Biology Interface and Chemical Mechanisms of Biological Systems. Current Chemical Biology covers the following areas: Chemical Synthesis (Syntheses of biologically important macromolecules including proteins, polypeptides, oligonucleotides, oligosaccharides etc.; Asymmetric synthesis; Combinatorial synthesis; Diversity-oriented synthesis; Template-directed synthesis; Biomimetic synthesis; Solid phase biomolecular synthesis; Synthesis of small biomolecules: amino acids, peptides, lipids, carbohydrates and nucleosides; and Natural product synthesis).