Vitamin D Inhibits Lipopolysaccharide (LPS)-Induced Inflammation in A549 Cells by Downregulating Inflammatory Cytokines

IF 1.5 Q3 MEDICINE, RESEARCH & EXPERIMENTAL
V. Gatera, R. Lesmana, I. Musfiroh, R. Judistiani, B. Setiabudiawan, R. Abdulah
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引用次数: 6

Abstract

Background Studies have shown that lung inflammation affects lung function, with life-threatening results. Vitamin D may play an important role in inhibiting inflammatory cytokines. Vitamin D deficiency is related to several lung problems, including respiratory distress syndrome, alveolar inflammation, epithelial damage, and hypoxia. Few studies have evaluated the benefits of vitamin D in preventing inflammation in alveolar cells. Material/Methods We developed a cell inflammation model induced by lipopolysaccharide (LPS) treatment. The effects of vitamin D on LPS-induced inflammation in A549 cells were examined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and the anti-inflammatory mechanism of vitamin D was evaluated using western blot analysis. Results Our results indicated that vitamin D promoted A549 cell survival following LPS-induced inflammation by downregulating nuclear factor nuclear factor kappa light chain enhancer of activated B cells, tumor necrosis factor-α, interleukin (IL)-1β, IL-6, and IL-12. Conclusions Our results indicated that vitamin D has the potential to manage lung inflammation, although further studies are needed.
维生素D通过下调炎性细胞因子抑制脂多糖(LPS)诱导的A549细胞炎症
研究表明,肺部炎症会影响肺功能,甚至危及生命。维生素D可能在抑制炎症细胞因子方面发挥重要作用。维生素D缺乏与几种肺部疾病有关,包括呼吸窘迫综合征、肺泡炎症、上皮损伤和缺氧。很少有研究评估维生素D在预防肺泡细胞炎症方面的益处。材料/方法建立脂多糖(LPS)诱导的细胞炎症模型。采用3-(4,5-二甲基噻唑-2-酰基)-2,5-二苯基溴化四唑(MTT)法检测维生素D对lps诱导的A549细胞的抗炎作用,采用western blot分析维生素D的抗炎机制。结果结果表明,维生素D通过下调活化B细胞的核因子、核因子kappa轻链增强子、肿瘤坏死因子-α、白细胞介素(IL)-1β、IL-6和IL-12,促进lps诱导炎症后A549细胞的存活。结论:我们的研究结果表明,维生素D有可能控制肺部炎症,尽管还需要进一步的研究。
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来源期刊
Medical Science Monitor Basic Research
Medical Science Monitor Basic Research MEDICINE, RESEARCH & EXPERIMENTAL-
CiteScore
6.00
自引率
0.00%
发文量
16
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