Dispersive solid phase microextraction based on aminefunctionalized bimodal mesoporous silica nanoparticles for separation and determination of calcium ions in chronic kidney disease

S. Davari, F. Hosseini, H. Shirkhanloo
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引用次数: 13

Abstract

The ultrasound assisted- dispersive solid phase microextraction method (USA-SPME) was used for in-vitro study on separation/extraction of calcium ions in human blood of chronic kidney disease (CKD). In this procedure, amine-functionalized bimodal mesoporous silica nanoparticle (NH2-UVM7) as a solid phase was used for in-vitro separation/extraction of calcium from blood/serum samples. Moreover, a mixture of NH2-UVM7 with ionic liquid and acetone (S/IL/Ac) was added to serum/blood sample containing of Ca (II) at pH of 7.3. After ultrasonic bath and centrifuging, NH2-UVM7/ IL settled down in bottom of tube, which was extracted Ca (II) ions by binding to amine group ([Ca]2+ →: NH2 ─ UVM7). The concentration of Ca (II) was determined by flame atomic absorption spectrometry (F-AAS, N2O, C2H2) after back extraction remained adsorbent in IL by 0.5 mL of HNO3 (0.5 M).
基于胺功能化双峰介孔二氧化硅纳米颗粒的分散固相微萃取分离和测定慢性肾脏疾病中的钙离子
采用超声辅助分散固相微萃取法(USA-SPME)对慢性肾脏疾病(CKD)患者血液中钙离子的分离提取进行了体外研究。在这个过程中,胺功能化的双峰介孔二氧化硅纳米颗粒(NH2-UVM7)作为固相用于体外分离/提取血/血清样品中的钙。将NH2-UVM7与离子液体和丙酮的混合物(S/IL/Ac)加入到pH为7.3的含Ca (II)的血清/血样中。超声浴和离心后,NH2-UVM7/ IL沉淀在管底,通过与胺基([Ca]2+→:NH2─UVM7)结合提取Ca (II)离子。采用火焰原子吸收光谱法(F-AAS, N2O, C2H2)测定Ca (II)的浓度,用0.5 mL HNO3 (0.5 M)反萃取后仍吸附在IL中。
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