The in-house monoclonal antibody against γ-globin chain; Thal N/B, accurately measured F cells in SEA-α thalassemia 1 trait

Journal of Immunoassay and Immunochemistry Pub Date : 2019-09-26 DOI:10.1080/15321819.2019.1671862

Lamplaimas Tangpan, Tippawadee Jansai, Sasiwan Kerdpoo, Tiemjan Kiewkarnkha, Ekthong Limweeraprajak, T. Tatu

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Abstract

ABSTRACT The monoclonal antibody (mAb) against γ-globin chain clone Thal N/B was produced. It was aimed at measuring the quantity of HbF (α2γ2)-containing red blood cells or F cells by flow cytometry. However, it may cross-react with Hb Bart’s (γ4) which is present in the SEA-α thalassemia 1 trait. We measured FC levels by flow cytometry using this in-house mAb for 100 blood samples. Prevalence of high FC trait in this cohort was 51%. Ten of 12 SEA-α thalassemia 1 trait were included in the 51% high FC individuals. Comparing FC levels in the 51 high FC individuals having and not having the SEA-α thalassemia 1 trait showed no difference of FC levels. It was concluded that Hb Bart’s did not interfere with FC measurement by flow cytometry using the in-house Thal N/B mAb. Therefore, it can be used for measuring F cell levels in regions having a high prevalence of the SEA-α thalassemia 1 trait. The findings of this research should apply to other clones of anti-γ-globin chain mAb that are aimed for HbF/FC quantification.

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抗γ-珠蛋白链单克隆抗体;Thal N/B，准确测定SEA-α地中海贫血1型性状中的F细胞

制备了抗γ-珠蛋白链克隆Thal N/B的单克隆抗体(mAb)。目的是用流式细胞术测定含HbF (α2γ2)的红细胞或F细胞的数量。然而，它可能与存在于SEA-α地中海贫血1性状中的Hb Bart 's (γ - 4)发生交叉反应。我们使用这种内部单抗对100份血液样本进行流式细胞术检测FC水平。该队列中高FC特征的患病率为51%。51%高FC个体中包含12个SEA-α地中海贫血1性状中的10个。比较51例具有和不具有SEA-α地中海贫血1型特征的高FC个体的FC水平，FC水平无差异。结论Hb Bart 's不干扰流式细胞术使用内部的Thal N/B单抗测定FC。因此，它可用于测量SEA-α地中海贫血1性状高流行地区的F细胞水平。本研究的发现应适用于其他针对HbF/FC定量的抗γ-珠蛋白链单抗克隆。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Journal of Immunoassay and Immunochemistry

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