Impact of smoking on frequency and spectrum of K-RAS and EGFR mutations in treatment naive Indonesian lung cancer patients

IF 5.1 Q1 ONCOLOGY
N. Masykura, J. Zaini, E. Syahruddin, S. Andarini, A. Hudoyo, Refniwita Yasril, A. Ridwanuloh, Heriawaty Hidajat, F. Nurwidya, A. Utomo
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引用次数: 7

Abstract

Background: Indonesia has the highest cigarette consumption in the world. We explored the clinical impact of smoking on the prevalence of EGFR and K-RAS mutations and survival in this prospective study. Methods: 143 treatment naive lung cancer patients were recruited from Persahabatan Hospital, a national tertiary hospital. DNA from cytological specimens had been extracted and genotyped for both EGFR and K-RAS mutations using a combination of PCR high resolution melting, restriction fragment length polymorphism (RFLP) and direct DNA sequencing. Results: EGFR mutation frequency in never smokers (NS) and ever smokers (ES) were 75% and 56% (p = 0.0401), respectively. In this cohort, the overall K-RAS mutation rate was 7%. Neither gender nor smoking history were associated with K-RAS mutation significantly. However, K-RAS transversion mutations were more common in male ES than transition mutations. Smoking history did not affect EGFR and K-RAS mutation frequencies in women. Concurrent EGFR/K-RAS mutation rate was 2.8% (4 of 143 patients). Four out of 91 EGFR mutation positive patients (4.4%) had simultaneous K-RAS mutation. Conclusions: In region where cigarette consumption is prevalent, smoking history affected frequencies of EGFR and K-RAS mutations, mainly in males.
吸烟对印尼初治肺癌患者K-RAS和EGFR突变频率和谱的影响
背景:印度尼西亚是世界上卷烟消费量最高的国家。在这项前瞻性研究中,我们探讨了吸烟对EGFR和K-RAS突变患病率和生存率的临床影响。方法:从国家三级医院Persahabatan医院招募143例初诊肺癌患者。从细胞学标本中提取DNA,并使用PCR高分辨率熔融、限制性片段长度多态性(RFLP)和直接DNA测序相结合的方法对EGFR和K-RAS突变进行基因分型。结果:从不吸烟者(NS)和曾经吸烟者(ES)的EGFR突变频率分别为75%和56% (p = 0.0401)。在该队列中,总体K-RAS突变率为7%。性别和吸烟史与K-RAS突变均无显著相关性。然而,K-RAS翻转突变在男性ES中比过渡突变更常见。吸烟史不影响女性EGFR和K-RAS突变频率。同时发生EGFR/K-RAS突变率为2.8%(143例患者中有4例)。91例EGFR突变阳性患者中有4例(4.4%)同时发生K-RAS突变。结论:在吸烟流行的地区,吸烟史影响EGFR和K-RAS突变的频率,主要发生在男性中。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
8.10
自引率
0.00%
发文量
10
审稿时长
16 weeks
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