Accumulation of phytoene and astaxanthin and related genes expression in Haematococcus pluvialis under sodium acetate stress

IF 1.3 4区生物学 Q3 MARINE & FRESHWATER BIOLOGY

Aquatic Biology Pub Date : 2020-11-19 DOI:10.3354/ab00733

X. Cong, X. Zang, M. Dong, Z. Wang, B. He, L. Hou, X. Wei, F. Zhang, M. Shang, Z. Yangzong, R. Li, X. Zhang

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引用次数: 5

Abstract

Phytoene and astaxanthin are 2 important carotenoids in the green alga Haematococcus pluvialis. Under environmental stress, the synthesis of phytoene in H. pluvialis increases significantly, and phytoene is converted to astaxanthin through enzymatic catalysis. This paper analyzes the relationship between astaxanthin and phytoene accumulation in carotenoid synthesis pathways under different concentrations of sodium acetate (NaAc) by high-performance liquid chromatography. The highest concentrations of phytoene and astaxanthin were observed at the NaAc concentration of 6 g l−1 on the 12th day of induction. The highest astaxanthin concentration achieved was 2.26 ± 0.28%. Therefore, we concluded that 6 g l−1 NaAc and induction for 12 d provided the optimal inducing conditions for astaxanthin accumulation in H. pluvialis. psy, pds, lcyB, β-carotene ketolase crtw, and crtz, which are genes related to phytoene and astaxanthin synthesis, were cloned and studied at the transcriptional level. crtw and crtz were continuously up-regulated since the first day of induction, while psy, pds, and lcyB were continuously up-regulated starting on the 3rd day of induction. These findings are important for enhancing our understanding of the mechanism of accumulation of phytoene and astaxanthin in H. pluvialis and provide a foundation for identifying the induction conditions necessary for optimizing astaxanthin production and increasing astaxanthin yields.

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醋酸钠胁迫下雨红球菌植物烯和虾青素积累及相关基因表达

植物烯和虾青素是绿藻雨红球藻中两种重要的类胡萝卜素。在环境胁迫下，雨水杨中植物烯的合成显著增加，植物烯通过酶催化转化为虾青素。采用高效液相色谱法分析了不同浓度乙酸钠(NaAc)作用下类胡萝卜素合成途径中虾青素与植物烯积累的关系。诱导第12天NaAc浓度为6 g l−1时，植物烯和虾青素含量最高。最高虾青素浓度为2.26±0.28%。综上所述，6 g l−1 NaAc诱导12 d为雨水杨虾青素积累的最佳诱导条件。克隆了植物烯和虾青素合成相关基因psy、pds、lcyB、β-胡萝卜素酮化酶crtw和crtz，并在转录水平上进行了研究。crtw、crtz自诱导第1天开始持续上调，而psy、pds、lcyB自诱导第3天开始持续上调。这些发现对于进一步了解雨水杨中植物烯和虾青素的积累机制具有重要意义，并为确定优化虾青素产量和提高虾青素产量所需的诱导条件提供了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Aquatic Biology 生物-海洋与淡水生物学

CiteScore

2.70

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： AB publishes rigorously refereed and carefully selected Feature Articles, Research Articles, Reviews and Notes, as well as Comments/Reply Comments (for details see MEPS 228:1), Theme Sections, Opinion Pieces (previously called ''As I See It'') (for details consult the Guidelines for Authors) concerned with the biology, physiology, biochemistry and genetics (including the ’omics‘) of all aquatic organisms under laboratory and field conditions, and at all levels of organisation and investigation. Areas covered include: -Biological aspects of biota: Evolution and speciation; life histories; biodiversity, biogeography and phylogeography; population genetics; biological connectedness between marine and freshwater biota; paleobiology of aquatic environments; invasive species. -Biochemical and physiological aspects of aquatic life; synthesis and conversion of organic matter (mechanisms of auto- and heterotrophy, digestion, respiration, nutrition); thermo-, ion, osmo- and volume-regulation; stress and stress resistance; metabolism and energy budgets; non-genetic and genetic adaptation. -Species interactions: Environment–organism and organism–organism interrelationships; predation: defenses (physical and chemical); symbioses. -Molecular biology of aquatic life. -Behavior: Orientation in space and time; migrations; feeding and reproductive behavior; agonistic behavior. -Toxicology and water-quality effects on organisms; anthropogenic impacts on aquatic biota (e.g. pollution, fisheries); stream regulation and restoration. -Theoretical biology: mathematical modelling of biological processes and species interactions. -Methodology and equipment employed in aquatic biological research; underwater exploration and experimentation. -Exploitation of aquatic biota: Fisheries; cultivation of aquatic organisms: use, management, protection and conservation of living aquatic resources. -Reproduction and development in marine, brackish and freshwater organisms