Kinetic Model of Enzymatic Hydrolysis of Protein about the Protein of Silkworm Pupae -- Alcalase System

Abstract

The silkworm pupa (SWP) is known for their nutritional value of high protein. In China, the production of protein of silkworm pupae (PSP) is very high because of the development of silk industry. This study applied the model which already was reported in the literature to the PSP - Alcalase system. It proved that the substrate inhibition existed in this system, and its reaction constants were determined as K_m=3.50 g·L^-1, k₂=251.7 min^-1, K_s=94.8 g·L^-1, k_d=41.4 min^-1, c₀=3.13×10^-4, r_max=16.2 g·L^-1·min^-1. At last, the experimental data were substituted this kinetic model, the average relative error was only 4.60%. So, it proved that kinetic model was reasonable, and the kinetic equation could model the hydrolysis process of PSP-Alcalase.