H. An, J. Yoo, M. K. Lee, M. Shin, G. Rhie, J. Seo, J. Chung, H. Eun, K. Cho
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引用次数: 12
Abstract
Purpose: To establish whether the effect of fractionating radiation modifies the effects of ultraviolet (UV) radiation on epidermal melanocytes, we compared the clinical and histological effects of single high dose radiation against repeated intermediate to low dose radiation on epidermal melanocytes.
Methods: Three minimal erythema UV doses (MED) were administered to three sites on the buttocks of healthy volunteers. One site was irradiated with 0.5 MED UV every day for 6 consecutive days, another site was irradiated with 1 MED UV every second day, and a third site received a single dose of radiation with 3 MED UV. The treatment was replicated on the other buttock. For the evaluation of UV-induced erythema and pigmentation, erythema and melanin indices were measured at 2 and 14 days post-irradiation. For purposes of histological evaluation, tissue specimens taken from each irradiated site at 2 and 14 days post-irradiation and were stained with monoclonal antibodies against Mel-5, HMB-45 and tyrosinase. Fontana-Masson silver staining, DOPA staining and split DOPA reactions were also performed.
Results: At 14 days post-irradiation, UV radiation induced melanocyte activation, proliferation and melanogenesis in proportion to the radiation dose administered to each fraction. The most prominent responses were observed after single high doses of radiation.
Conclusion: When the total administered dose is identical, fractionation of radiation dose diminishes the effects of UV radiation on epidermal melanocytes. Furthermore, long, uninterrupted doses of UV radiation were found to more effective in inducing melanogenesis and melanocyte activation.
目的:为了确定分次辐射是否改变了紫外线(UV)辐射对表皮黑色素细胞的影响,我们比较了单次高剂量辐射和多次中、低剂量辐射对表皮黑色素细胞的临床和组织学影响。方法:对健康志愿者臀部3个部位施用3次微红斑紫外线剂量(MED)。一个部位每天接受0.5 MED UV照射,连续6天,另一个部位每隔一天接受1 MED UV照射,第三个部位接受3 MED UV单剂量照射。在另一个臀部也进行了同样的治疗。在照射后第2天和第14天测量红斑和黑色素指数,以评估紫外线诱导的红斑和色素沉着。为了进行组织学评估,在照射后2天和14天从每个照射部位取组织标本,并用Mel-5、HMB-45和酪氨酸酶单克隆抗体进行染色。同时进行Fontana-Masson银染色、DOPA染色和分裂DOPA反应。结果:在照射后14天,紫外线辐射诱导黑素细胞活化、增殖和黑素形成与给药剂量成正比。在单次高剂量辐射后观察到最显著的反应。结论:在给药总剂量相同的情况下,照射剂量的分段减小了紫外线照射对表皮黑色素细胞的影响。此外,长时间不间断的紫外线辐射被发现更有效地诱导黑素生成和黑素细胞活化。