An Efficient Protocol for Micro Propagation of Ginger (Zingiber Officinale Rosc.) cv. Volvo via Sprout Bud Culture

Abstract

An efficient, simple micro propagation method was developed for Zingiber officinale Rosc.) cv. Volvo using fresh sprouting bud in basal culture media. Explants were cultured on to Murashige and Skoog’s (MS) medium supplemented with different concentrations and combinations of BA (Benzyl adenine) and KIN (Kinetin) for shoot micro propagation and IBA (Indol-3-Butyric acid) and NAA (α- Naphthalene acetic acid) for root induction. Explants cultured on MS basal medium supplemented with 2.0 mg/L BA + 0.5gm/L KIN gave the highest averages in shoot number (11.3 shoots/explant), leaf number (22.8 leaves/shoot) and highest plant height (16.4 cm) and followed by the concentration 2 mg/L of BA and 1mg/L KIN gave the highest averages of shoot number (9.8 shoots/explant), leaf number (13.9 leaves/shoot) and highest plant height (10.3 cm). In vitro shootlets were rooted on to the half strength MS basal media supplemented with the 1.0 mg/L NAA and followed by 1mg/L IBA and 2mg/L NAA obtained the highest averages of roots number and roots length were achieved (9.6 roots/shoot and 11.1 cm respectively) and (8.3 roots/shoot and highest length of roots 10.8 cm respectively. Rooted shoots were transplanted in the green house for hardening off planted at the mixture of forest soil, coffee husk and sand soil (2:1:1) ratio respectively and their survival was 96% in the field condition. Hence, the acclimatization seedlings were set edible rhizomes in cement bug and pot in seven months.